来自中国铁线莲根的三萜皂苷。

Triterpenoid saponins from the roots of Clematis chinensis Osbeck.

作者信息

Liu Li-Fang, Ma Xiao-Li, Wang Yu-Xin, Li Feng-Wen, Li Yun-Man, Wan Zhu-Qing, Tang Qiu-Ling

机构信息

The Key Laboratory of Modern Chinese Medicines (Ministry of Education) and Department of Pharmacognosy, China Pharmaceutical University, Nanjing, China.

出版信息

J Asian Nat Prod Res. 2009;11(5):389-96. doi: 10.1080/10286020902867268.

DOI:10.1080/10286020902867268

PMID:19504380

Abstract

A new triterpenoid saponin named clematichinenoside AR(2), along with the six known compounds, was isolated and characterized from Clematis chinensis Osbeck (Ranunculaceae), a commonly used traditional Chinese medicine with anti-inflammatory and anti-rheumatoid activities. The structure of the new saponin was elucidated as 3-O-beta-[(O-alpha-L-rhamnopyranosyl-(1 --> 6)-O-beta-D-glucopyranosyl-(1 --> 4)-O-beta-D-glucopyranosyl-(1 --> 4)-O-beta-D-ribopyranosyl-(1 --> 3)-O-alpha-L-rhamnopyranosyl-(1 --> 2)-alpha-L-arabinopyranosyl)oxy]olean-12-en-21alpha-hydroxy-28-oic acid-O-alpha-L-rhamnopyranosyl-(1 --> 4)-O-beta-D-glucopyranosyl-(1 --> 6)-beta-D-glucopyranosyl ester (1) by spectral analysis and chemical methods. The effects of two major saponins (clematichinenosides AR and AR(2)) on the secretion of TNF-alpha in murine peritoneal macrophages induced by lipopolysaccharides were further investigated. The result indicated that a majority of triterpenoid saponins of this herb may be useful in the exploration of lead compounds for the treatment of some autoimmune diseases.

摘要

从常用的具有抗炎和抗类风湿活性的中药毛茛科植物威灵仙（Clematis chinensis Osbeck）中分离并鉴定出一种名为威灵仙新苷AR(2)的新三萜皂苷以及六种已知化合物。通过光谱分析和化学方法确定该新皂苷的结构为3-O-β-[(O-α-L-鼠李吡喃糖基-(1→6)-O-β-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃核糖基-(1→3)-O-α-L-鼠李吡喃糖基-(1→2)-α-L-阿拉伯吡喃糖基)氧基]齐墩果-12-烯-21α-羟基-28-酸-O-α-L-鼠李吡喃糖基-(1→4)-O-β-D-吡喃葡萄糖基-(1→6)-β-D-吡喃葡萄糖基酯（1）。进一步研究了两种主要皂苷（威灵仙新苷AR和AR(2)）对脂多糖诱导的小鼠腹腔巨噬细胞中肿瘤坏死因子-α分泌的影响。结果表明，该草药中的大多数三萜皂苷可能有助于探索治疗某些自身免疫性疾病的先导化合物。