瑞香狼毒中新的黄酮 C-糖苷。

A new flavone C-glycoside from Clematis rehderiana.

机构信息

State Key Laboratory of Phytochemistry and Plant Resources of West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.

出版信息

Molecules. 2010 Jan 29;15(2):672-9. doi: 10.3390/molecules15020672.

DOI:10.3390/molecules15020672

PMID:20335937

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6263201/

Abstract

A new flavone C-glycoside, isovitexin 6''-O-E-p-coumarate (1) and two known flavonoid glycosides--quercetin 3-O-beta-D-glucuronopyranoside (2) and isoorientin (3)--were isolated from an ethanol extract of aerial parts of Clematis rehderiana. Their structures were determined by spectroscopic methods. The antioxidant effects of the two flavone C-glycosides were evaluated by both the MTT and DPPH assays. Compound 1 showed potent activities against H2O2-induced impairment in PC12 cells within the concentration range tested, whereas compound 3 scavenged DPPH radical strongly, with an IC50 value of 13.5 microM.

摘要

从Clematis rehderiana 的地上部分的乙醇提取物中分离得到一种新的类黄酮 C-糖苷，异荭草素 6'-O-E-对香豆酸酯（1）和两种已知的类黄酮糖苷--槲皮素 3-O-β-D-葡萄糖醛酸吡喃糖苷（2）和异荭草素（3）。通过光谱方法确定了它们的结构。通过 MTT 和 DPPH 测定法评估了两种类黄酮 C-糖苷的抗氧化作用。在测试浓度范围内，化合物 1 对 H2O2 诱导的 PC12 细胞损伤表现出很强的活性，而化合物 3 对 DPPH 自由基具有很强的清除作用，IC50 值为 13.5 μM。