Grössner-Schreiber Birte, Teichmann Jan, Hannig Matthias, Dörfer Christof, Wenderoth Dirk F, Ott Stephan J
Department of Operative Dentistry and Periodontology, School of Dentistry, University of Kiel, Kiel, Germany.
Clin Oral Implants Res. 2009 Aug;20(8):817-26. doi: 10.1111/j.1600-0501.2009.01729.x. Epub 2009 Jun 7.
Plaque accumulation on implant surfaces can result in peri-implantitis with potential implant loss. The aim of the present study was to examine the influence of zirconium nitride (ZrN) as a potential implant surface on the biofilm composition and diversity in vivo.
ZrN- or titanium (Ti)-coated glass specimens and ZrN or roughened Ti discs were used as substrates. Pure glass and polished titanium served as controls. The specimens were mounted on removable intraoral splints in five adults. After 24 h of intraoral exposure, the biofilms were analyzed applying single-strand conformation polymorphism (SSCP analysis) of 16S rRNA genes. Sequence analysis of the dominant bands excised from the SSCP fingerprints allowed to taxonomically describe bacteria derived from biofilm samples.
The highest number of bands was counted on pure glass and Ti 800. ZrN-coated glass and ZrN-coated titanium discs showed the lowest values for species richness. However, no significant differences were observed regarding the diversity of the identified bacterial species among all the surfaces examined. A total of 46 different bacteria were identified. The dominant bands within the fingerprints indicated bacteria belonging to the Streptococcus group as identified by their 16S rDNA sequence.
A coating of glass surfaces with ZrN significantly reduced the species richness in early bacterial colonization but the diversity was not significantly changed. In consideration of the results obtained by this and former studies a ZrN coating appears to rather modify the quantity of early bacterial adherence than the quality of the microbial community structure.
种植体表面的菌斑堆积可导致种植体周围炎，并可能导致种植体脱落。本研究的目的是研究氮化锆（ZrN）作为一种潜在的种植体表面对体内生物膜组成和多样性的影响。
使用涂有ZrN或钛（Ti）的玻璃标本以及ZrN或粗糙化的Ti盘作为底物。纯玻璃和抛光钛作为对照。将标本安装在五名成年人的可摘口腔夹板上。口腔暴露24小时后，应用16S rRNA基因的单链构象多态性（SSCP分析）对生物膜进行分析。对从SSCP指纹图谱中切下的优势条带进行序列分析，从而对生物膜样本中的细菌进行分类描述。
在纯玻璃和Ti 800上计数的条带数量最多。涂有ZrN的玻璃和涂有ZrN的钛盘的物种丰富度值最低。然而，在所有检查的表面中，所鉴定细菌物种的多样性没有观察到显著差异。总共鉴定出46种不同的细菌。指纹图谱中的优势条带表明细菌属于链球菌属，这是通过它们的16S rDNA序列鉴定出来的。
用ZrN涂覆玻璃表面可显著降低早期细菌定植中的物种丰富度，但多样性没有显著变化。考虑到本研究和以前研究获得的结果，ZrN涂层似乎更多地改变了早期细菌粘附的数量，而不是微生物群落结构的质量。
