Department of Environmental Health Sciences, University Medical Center, Freiburg, Germany.
Int J Oral Maxillofac Implants. 2012 Jul-Aug;27(4):811-9.
The aim of the present in vivo study was to examine alterations of the microbial community structure in biofilms on different dental implant surfaces over the time.
Zirconium nitride-coated glass (ZrN-glass) and ZrN-coated polished titanium (ZrN-Ti) disks were used as substrates and polished titanium (Ti-pol) was used as a control. The specimens were mounted on removable intraoral splints in one adult. After 24 hours and 14 days of intraoral exposure, the microbial biofilms were analyzed by generating 16S rRNA gene clone libraries.
ZrN coating of a Ti surface altered the microbial composition early on (24 hours), with a tendency to augment Lactobacillus-related phylotypes later. Long-term exposure (14 days) of dental implant surfaces to microbes resulted in a significantly different composition of the biofilm on all three tested surfaces.
This preliminary study showed that a ZrN-Ti disk surface harbors a significantly different microbial composition from a polished Ti surface. Further improvement of ZrN physical vapor deposition coatings might help to influence the adhesion of bacteria that are less pathogenic, thereby reducing the risk of peri-implantitis.
本体内研究旨在随着时间的推移，研究不同牙种植体表面生物膜中微生物群落结构的变化。
氮化锆涂层玻璃（ZrN-玻璃）和氮化锆涂层抛光钛（ZrN-Ti）圆盘被用作基底，抛光钛（Ti-pol）被用作对照。将标本安装在一个成年人的可移动口腔夹板上。在口腔内暴露 24 小时和 14 天后，通过生成 16S rRNA 基因克隆文库来分析微生物生物膜。
Ti 表面的 ZrN 涂层早期改变了微生物的组成（24 小时），随后倾向于增加与乳杆菌相关的分类群。牙种植体表面微生物的长期暴露（14 天）导致所有三种测试表面的生物膜组成明显不同。
这项初步研究表明，ZrN-Ti 盘表面的微生物组成与抛光 Ti 表面明显不同。进一步改进 ZrN 物理气相沉积涂层可能有助于影响粘附性较低的细菌，从而降低种植体周围炎的风险。
