Yoshimoto Y, Wolfsen A R, Odell W D
Science. 1977 Aug 5;197(4303):575-7. doi: 10.1126/science.195341.
By means of two assay systems, a beta chain human chorionic gonadotropin radioimmunoassay and a radioreceptor gonadotropin assay, a chorionic gonadotropin-like substance was demonstrated in extracts of liver and colon obtained at autopsy from three patients who died of nonneoplastic disease. In contrast to placental chorionic gonadotropin, colon and liver chorionic gonadotropin was not bound to concanavalin A-Sepharose columns, indicating that this substance possessed little or no carbohydrate. Previous workers demonstrated that desialylated human chorionic gonadotropin possesses little or no bioactivity in vivo but retains full radioreceptor and radioimmunoassay activity in vitro. Our data suggest that the genome responsible for the human chorionic gonadotropin production is not completely suppressed in adult nonendocrine tissues, and that the chorionic gonadotropin produced by colon and liver has little or no bioactivity in vivo because of its low carbohydrate content. Since many normal tissues produce chorionic gonadotropin, bioactivity may be modulated by regulation of carbohydrate content.
通过两种检测系统，即β链人绒毛膜促性腺激素放射免疫测定法和促性腺激素放射受体测定法，在三名死于非肿瘤性疾病患者尸检时获得的肝脏和结肠提取物中证实了一种绒毛膜促性腺激素样物质。与胎盘绒毛膜促性腺激素不同，结肠和肝脏的绒毛膜促性腺激素不与伴刀豆球蛋白A-琼脂糖柱结合，这表明该物质几乎不含有或不含有碳水化合物。先前的研究人员表明，去唾液酸人绒毛膜促性腺激素在体内几乎没有生物活性，但在体外仍保留完全的放射受体和放射免疫测定活性。我们的数据表明，负责产生人绒毛膜促性腺激素的基因组在成人非内分泌组织中并未完全被抑制，并且结肠和肝脏产生的绒毛膜促性腺激素由于其低碳水化合物含量在体内几乎没有生物活性。由于许多正常组织都会产生绒毛膜促性腺激素，生物活性可能通过碳水化合物含量的调节来调节。
