Bruce Alison, Pacey Ian E, Dharni Poonam, Scally Andy J, Barrett Brendan T
Bradford School of Optometry and Vision Science, University of Bradford, Bradford, UK.
Open Ophthalmol J. 2009 Apr 20;3:10-4. doi: 10.2174/1874364100903010010.
To evaluate repeatability and reproducibility of macular thickness measurements in visually normal eyes using the Topcon 3D OCT-1000.
Phase 1 investigated scan repeatability, the effect of age and pupil dilation. Two groups (6 younger and 6 older participants) had one eye scanned 5 times pre and post- dilation by 1 operator. Phase 2 investigated between-operator, within and between-visit reproducibility. 10 participants had 1 un-dilated eye scanned 3 times on 2 separate visits by 2 operators.
PHASE 1: No significant difference existed between repeat scans (p=0.75) and no significant difference was found pre- and post-dilation (p=0.54). In the younger group variation was low (95% limits +/- 3.62microm) and comparable across all retinal regions. The older group demonstrated greater variation (95% limits +/- 7.6microm). PHASE 2: For a given retinal location, 95% confidence limits for within-operator, within-visit reproducibility was 5.16microm. This value increased to 5.56microm for the same operator over two visits and to 6.18microm for two operators over two visits.
A high level repeatability, close to 6microm, of macular thickness measurement is possible using the 3D OCT- 1000. Measured differences in macular thickness between successive visits that exceed 6microm in pre-presbyopic individuals are therefore likely to reflect actual structural change. OCT measures are more variable in older individuals and it is advisable to take a series of scans so that outliers can be more easily identified.
使用拓普康3D OCT - 1000评估视力正常眼睛黄斑厚度测量的可重复性和再现性。
第一阶段研究扫描可重复性、年龄和瞳孔散大的影响。两组（6名年轻参与者和6名年长参与者）由1名操作人员在散瞳前后对一只眼睛进行5次扫描。第二阶段研究操作人员间、同一检查者不同时间及不同检查者间的再现性。10名参与者由2名操作人员在2次单独检查中对1只未散瞳的眼睛进行3次扫描。
第一阶段：重复扫描之间无显著差异（p = 0.75），散瞳前后也无显著差异（p = 0.54）。年轻组的变异较低（95%界限值±3.62微米），且在所有视网膜区域相当。年长组显示出更大的变异（95%界限值±7.6微米）。第二阶段：对于给定的视网膜位置，同一检查者同一时间的可重复性95%置信区间为5.16微米。同一检查者两次检查时该值增至5.56微米，两名检查者两次检查时增至6.18微米。
使用3D OCT - 1000进行黄斑厚度测量可达到接近6微米的高重复性。因此，在未老视个体中，连续检查之间黄斑厚度测量差异超过6微米很可能反映实际结构变化。在年长个体中OCT测量的变异性更大，建议进行一系列扫描以便更容易识别异常值。
