Abdelsalam Nader R, Abdel-Megeed Ahmed, Ghareeb Rehab Y, Ali Hayssam M, Salem Mohamed Z M, Akrami Mohammad, Al-Hayalif Muwafaq F A, Desoky El-Sayed M
Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, 21531 Alexandria, Egypt.
Department of Plant Protection, Faculty of Agriculture (Saba Basha), Alexandria University, 21531 Alexandria, Egypt.
Saudi J Biol Sci. 2022 Apr;29(4):2306-2313. doi: 10.1016/j.sjbs.2021.11.059. Epub 2021 Dec 3.
Nanoparticles have a positive impact in several subjects especially in agriculture, while their safety is still being debated. Numerous commercial nano pesticide, insecticides, and fertilizers products are found in the local markets without any intensely studies on the side effect of these products on plant, human as well as environmental effects. The present study aimed to evaluate the genotoxicity of commercial amino zinc nanoparticles (AZ NPs) on L. during seeds germination and root elongation using concentration ranges (50, 100, and 150 ppm) at different exposure times (8, 16 and 24 hrs). Long term exposure to AZ NPs, exhibited only slight variation in germination rates and the elongation of roots was affected by AZ NPs treatment ranged from 97.66 to 100%. Significant reduction in the mitotic index was 35.33% after 24 hrs and 150 ppm of AZ NPs, was also observed comparing with control which was 88.0%. Genotoxicity was evaluated at a cytological level in root meristems that revealed sever variations in mitotic activity, chromosomal aberrations, and micronuclei release. Results exhibited that nano amino zinc could enter effortlessly into the cells and inhibit the normal cellular function. The decrease in the emergence of chromosomal aberrations resulting from AZ NPs exposure in a dose-dependent manner was clearly indicated that AZ NPs has induced genotoxic effect on wheat root tips.
纳米颗粒在多个领域尤其是农业中具有积极影响，但其安全性仍存在争议。在当地市场上发现了许多商业纳米农药、杀虫剂和肥料产品，却没有对这些产品对植物、人类以及环境的副作用进行任何深入研究。本研究旨在评估商业氨基锌纳米颗粒（AZ NPs）在不同暴露时间（8、16和24小时）使用浓度范围（50、100和150 ppm）对L. during种子萌发和根伸长的遗传毒性。长期暴露于AZ NPs，发芽率仅表现出轻微变化，根伸长受到AZ NPs处理的影响，范围为97.66%至100%。与对照（88.0%）相比，在24小时和150 ppm的AZ NPs处理后，有丝分裂指数显著降低至35.33%。在根分生组织的细胞学水平上评估遗传毒性，结果显示有丝分裂活性、染色体畸变和微核释放存在严重变化。结果表明，纳米氨基锌可以轻松进入细胞并抑制正常细胞功能。AZ NPs暴露导致染色体畸变出现的剂量依赖性降低清楚地表明，AZ NPs对小麦根尖具有遗传毒性作用。
