Hendriksen Rene S, Mikoleit Matthew, Carlson Valeria P, Karlsmose Susanne, Vieira Antonio R, Jensen Arne B, Seyfarth Anne Mette, DeLong Stephanie M, Weill François-Xavier, Lo Fo Wong Danilo Marino Armando, Angulo Frederick J, Wegener Henrik C, Aarestrup Frank M
National Food Institute, WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens, and Community Reference Laboratory for Antimicrobial Resistance, Technical University of Denmark, Copenhagen, Denmark.
J Clin Microbiol. 2009 Sep;47(9):2729-36. doi: 10.1128/JCM.02437-08. Epub 2009 Jul 1.
An international external quality assurance system (EQAS) for the serotyping of Salmonella species was initiated in 2000 by WHO Global Salm-Surv to enhance the capacity of national reference laboratories to obtain reliable data for surveillance purposes worldwide. Seven EQAS iterations were conducted between 2000 and 2007. In each iteration, participating laboratories submitted serotyping results for eight Salmonella isolates. A total of 249 laboratories in 96 countries participated in at least one EQAS iteration. A total of 756 reports were received from the participating laboratories during the seven EQAS iterations. Cumulatively, 76% of participating laboratories submitted data for all eight strains, and 82% of strains were correctly serotyped. In each iteration, 84% to 96% of the laboratories correctly serotyped the Salmonella enterica serovar Enteritidis isolate that was included as an internal quality control strain. Regional differences in performance were observed, with laboratories in Central Asia and the Middle East performing less well overall than those in other regions. Errors that resulted in incorrect serovar identification were typically caused by difficulties in the detection of the phase two flagellar antigen or in differentiation within antigen complexes; some of these errors are likely related to the quality of the antisera available. The results from the WHO Global Salm-Surv EQAS, the largest of its kind in the world, show that most laboratories worldwide are capable of correctly serotyping Salmonella species. However, this study also indicates a continuing need for improvement. Future training efforts should be aimed at enhancing the ability to detect the phase two flagellar antigen and at disseminating information on where to purchase high-quality antisera.
2000年，世界卫生组织全球沙门氏菌监测项目启动了一项针对沙门氏菌血清分型的国际外部质量保证体系（EQAS），以提高国家参考实验室获取全球监测可靠数据的能力。2000年至2007年期间进行了七次EQAS迭代。在每次迭代中，参与实验室提交了八株沙门氏菌分离株的血清分型结果。96个国家的249个实验室至少参与了一次EQAS迭代。在七次EQAS迭代期间，共收到参与实验室的756份报告。累计而言，76%的参与实验室提交了所有八株菌株的数据，82%的菌株血清分型正确。在每次迭代中，84%至96%的实验室对作为内部质量控制菌株纳入的肠炎沙门氏菌血清型肠炎亚种分离株进行了正确的血清分型。观察到了性能上的区域差异，中亚和中东地区的实验室总体表现不如其他地区的实验室。导致血清型鉴定错误的误差通常是由检测第二相鞭毛抗原或抗原复合物内的鉴别困难引起的；其中一些误差可能与可用抗血清的质量有关。世界卫生组织全球沙门氏菌监测EQAS是世界上同类项目中规模最大的，其结果表明，全球大多数实验室都能够对沙门氏菌进行正确的血清分型。然而，这项研究也表明仍有持续改进的需求。未来的培训工作应旨在提高检测第二相鞭毛抗原的能力，并传播有关购买高质量抗血清地点的信息。
