酿酒酵母对甲磺酸甲酯敏感突变体的分离与鉴定。
Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae.
作者信息
Prakash L, Prakash S
出版信息
Genetics. 1977 May;86(1):33-55. doi: 10.1093/genetics/86.1.33.
Abstract
We have isolated mutants sensitive to methyl methanesulfonate (MMS) in Saccharomyces cerevisiae. Alleles of rad1, rad4, rad52, rad55 and rad57 were found amoung these mms mutants. Twenty-nine of the mms mutants which complement the existing radiation-sensitive (rad and rev) mutants belong to 22 new complementation groups. Mutants from five complementation groups are sensitive only to MMS. Mutants of 11 complementation groups are sensitive to UV or X rays in addition to MMS, mutants of six complementation groups are sensitive to all three agents. The cross-sensitivities of these mms mutants to UV and X rays are discussed in terms of their possible involvement in DNA repair. Sporulation is reduced or absent in homozygous diploids of mms mutants from nine complementation groups.
摘要
我们在酿酒酵母中分离出了对甲磺酸甲酯（MMS）敏感的突变体。在这些MMS突变体中发现了rad1、rad4、rad52、rad55和rad57的等位基因。29个能与现有的辐射敏感（rad和rev）突变体互补的MMS突变体属于22个新的互补群。来自五个互补群的突变体仅对MMS敏感。11个互补群的突变体除对MMS敏感外，还对紫外线或X射线敏感，6个互补群的突变体对所有三种诱变剂均敏感。根据这些MMS突变体可能参与DNA修复的情况，讨论了它们对紫外线和X射线的交叉敏感性。来自九个互补群的MMS突变体的纯合二倍体的孢子形成减少或缺失。
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