Salamanca M J, Fernández N, Cesar A, Antón R, Lopez P, Delvalls A
Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Polígono Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain.
Ecotoxicology. 2009 Nov;18(8):1051-7. doi: 10.1007/s10646-009-0378-2. Epub 2009 Jul 10.
Sediments usually contain many contaminants derived from human activities. In case of dredging activities, these sediment-bound contaminants arise following the excavation and remobilization of sediments. Previous studies have used different species of clam, crabs, lugworms, etc. for the evaluation of dredged material in situ, but there are not studies that use acute bioassays for these purposes. The sea-urchin embryo bioassay has been chosen to characterize biological effects in situ in two ports of the southwest of Spain, the Port of Huelva and the Port of Cadiz. The sea-urchin embryo bioassay has been adapted for in situ evaluation of seawater quality in coastal areas, however, they are necessary for further improvements to take into account differences of temperature between sites. This temperature variation is one of the principal reasons (other than pollution) of larval mortality and the slow down in the growth rate of the urchin. In the present study a bioassay was conducted in both field and laboratory conditions, in order to compare the effects in situ with the effects under controlled conditions of temperature, salinity and oxygen dissolved. Results showed a good correlation between samples obtained in situ and in the laboratory, but in the field the percentage of normal pluteus larvae is less than under laboratory conditions.
沉积物通常含有许多源自人类活动的污染物。在疏浚活动中，这些与沉积物结合的污染物会随着沉积物的挖掘和再迁移而出现。先前的研究使用了不同种类的蛤、蟹、沙蚕等对疏浚材料进行现场评估，但尚无使用急性生物测定法进行此类评估的研究。已选择海胆胚胎生物测定法来表征西班牙西南部两个港口（韦尔瓦港和加的斯港）的现场生物效应。海胆胚胎生物测定法已被用于沿海地区海水质量的现场评估，然而，有必要进一步改进以考虑不同地点之间的温度差异。这种温度变化是幼虫死亡以及海胆生长速度减缓的主要原因之一（除污染外）。在本研究中，在野外和实验室条件下都进行了生物测定，以便将现场效应与温度、盐度和溶解氧受控条件下的效应进行比较。结果表明，现场采集的样本与实验室样本之间具有良好的相关性，但在野外，正常长腕幼虫的百分比低于实验室条件下的百分比。
