Affonso Vanessa Ribeiro, Bizzo Humberto Ribeiro, Lage Celso Luiz Salgueiro, Sato Alice
Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, CCS, Bloco G, Cidade Universitaria, Ilha do Fundao, 21952-590 Rio de Janeiro, Brazil.
J Agric Food Chem. 2009 Jul 22;57(14):6392-5. doi: 10.1021/jf900816c.
In vitro shoots of thyme (Thymus vulgaris L.) were established, and the effects of the auxin indole-3-acetic (IAA) acid and the cytokinins benzyladenine (BA), zeatin (ZEA), and kinetin (KIN) at 1.0, 5.0, and 10.0 microM on rooting, biomass production, and volatile compounds production by these plants were investigated. The volatiles were extracted by solid phase microextraction (SPME) and analyzed by gas chromatography. The highest biomass shoot growth was obtained with BA at 5.0 microM, while IAA at all concentrations tested achieved 100% rooting frequency. The three major compounds were gamma-terpinene (22.8-38.8%), p-cymene (13.8-27.9%), and thymol (6.5-29.0%). Quantitative changes of these compounds were observed in response to the effect of varying growth regulators concentrations in the culture medium. Growing Thymus vulgaris L. plants in media supplemented with IAA at 1.0 microM increased volatile compounds such as thymol by 315%. Nevertheless, the same major compounds were produced in all treatments and no qualitative changes were observed in the volatile profile of thyme plants.
建立了百里香（Thymus vulgaris L.）的离体芽，并研究了生长素吲哚 - 3 - 乙酸（IAA）以及细胞分裂素苄基腺嘌呤（BA）、玉米素（ZEA）和激动素（KIN）在1.0、5.0和10.0微摩尔浓度下对这些植物生根、生物量生产和挥发性化合物产生的影响。挥发性化合物通过固相微萃取（SPME）提取，并通过气相色谱进行分析。在5.0微摩尔的BA处理下获得了最高的地上部生物量生长，而在所有测试浓度下的IAA都实现了100%的生根频率。三种主要化合物为γ - 萜品烯（22.8 - 38.8%）、对伞花烃（13.8 - 27.9%）和百里香酚（6.5 - 29.0%）。观察到这些化合物的定量变化是对培养基中不同生长调节剂浓度效应的响应。在添加1.0微摩尔IAA的培养基中培养百里香植物，使百里香酚等挥发性化合物增加了315%。然而，所有处理中都产生了相同的主要化合物，并且百里香植物的挥发性成分没有观察到定性变化。
