Hamden Khaled, Carreau Serge, Jamoussi Kamel, Miladi Slaheddine, Lajmi Samiha, Aloulou Dorra, Ayadi Fatma, Elfeki Abdelfattah
Laboratry of Animal Ecophysiology, Department of Life Science, Faculty of Science, University of Sfax, Tunisia.
J Nutr Sci Vitaminol (Tokyo). 2009 Jun;55(3):215-22. doi: 10.3177/jnsv.55.215.
Diabetes mellitus is a major endocrine disorder and a growing health problem in most countries which can be ameliorated by numerous bio-molecules such as 1alpha,25 dihydroxyvitamin D3 [1alpha,25(OH)2VD3]. With this in mind, the current study investigated the therapeutic and preventive effects of 1alpha,25(OH)2VD3 on diabetes and its side effects: toxicity in liver, pancreas and kidneys. Our results show that administration of 1alpha,25(OH)2VD3 in diabetic rats increased the plasmatic insulin level, favored the normal blood glucose levels and normalized the hepatic glycogen concentration. In addition, 1alpha,25(OH)2VD3 enhanced superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) (by 207, 52 and 72%, respectively) compared to diabetic rats. It also reduced lipid peroxidation and the indices of toxicity in liver and kidneys by significantly decreasing alkaline phosphatases (PAL), aspartate and lactate transaminase (AST and ALT) activities, total and direct bilirubin, triglycerides (TG), cholesterol, creatinine, urea and iron levels in diabetic rats. Moreover, the plasmatic non-enzymatic antioxidant level of HDL-cholesterol, magnesium (Mg), calcium (Ca) and copper (Cu) increased after 1alpha,25(OH)2VD3 administration. The administration of 1alpha,25(OH)2VD3 in diabetic rats protects against alloxan-induced histological changes in pancreas.
from these data, it is concluded that 1alpha,25(OH)2VD3 might be useful for the therapy and prevention of diabetes and the numerous side effects especially toxicity in liver, pancreas and kidneys and this protective effect is more obvious in our preventive experiment.
糖尿病是一种主要的内分泌紊乱疾病，在大多数国家，它作为一个日益严重的健康问题，可通过多种生物分子（如1α,25 - 二羟基维生素D3 [1α,25(OH)2VD3]）得到改善。基于此，本研究调查了1α,25(OH)2VD3对糖尿病及其副作用（肝脏、胰腺和肾脏毒性）的治疗和预防作用。我们的结果表明，给糖尿病大鼠施用1α,25(OH)2VD3可提高血浆胰岛素水平，有利于血糖水平正常化，并使肝糖原浓度恢复正常。此外，与糖尿病大鼠相比，1α,25(OH)2VD3使超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）分别提高了207%、52%和72%。它还通过显著降低糖尿病大鼠的碱性磷酸酶（PAL）、天冬氨酸和乳酸转氨酶（AST和ALT）活性、总胆红素和直接胆红素、甘油三酯（TG）、胆固醇、肌酐、尿素和铁水平，减少了脂质过氧化以及肝脏和肾脏的毒性指标。此外，施用1α,25(OH)2VD3后，血浆中高密度脂蛋白胆固醇、镁（Mg）、钙（Ca）和铜（Cu）的非酶抗氧化水平有所提高。给糖尿病大鼠施用1α,25(OH)2VD3可预防四氧嘧啶诱导的胰腺组织学变化。
从这些数据可以得出结论，1α,25(OH)2VD3可能对糖尿病的治疗和预防以及众多副作用（尤其是肝脏、胰腺和肾脏的毒性）有用，并且这种保护作用在我们的预防实验中更为明显。
