Lestari Shofiyah Ika, Han Feifei, Wang Fei, Ge Beilei
Department of Food Science, 111 Food Science Building, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, USA.
J Food Prot. 2009 Jun;72(6):1165-72. doi: 10.4315/0362-028x-72.6.1165.
In this 1-year survey from October 2006 to September 2007, we isolated and characterized 126 Salmonella isolates from conventionally raised (n=141) and organically raised (n=53) chicken carcasses obtained from 27 retail stores in Baton Rouge, Louisiana. Salmonella was isolated from 22% of conventional and from 20.8% of organic chicken samples. Eight Salmonella serovars were identified; predominant ones included Kentucky, Hadar, and Enteritidis. The vast majority of isolates within the same chicken sample possessed the same pulsed-field gel pattern. All Salmonella isolates were susceptible to amikacin, ceftriaxone, and ciprofloxacin; however, decreased susceptibility to quinolones (7.1%) or extended-spectrum cephalosporins (45.2%) was observed. Resistance to multiple antimicrobials (two or more) was found among 52.4% of the Salmonella isolates. Antimicrobial resistance profiles differed greatly among Salmonella serovars and also depended on the type of chicken from which they were recovered. Salmonella Kentucky isolates from organic chicken samples were susceptible to 11 of the antimicrobials tested, whereas those from conventional chickens were only susceptible to 4 antimicrobials. Three Salmonella Kentucky isolates from conventional chickens possessed multidrug resistance phenotype MDR-AmpC. Results of this study provide baseline data on both prevalence and antimicrobial resistance of Salmonella in retail chickens in this region and emphasize the need for implementing effective control measures to reduce Salmonella contamination and the levels of antimicrobial-resistant Salmonella in both conventionally and organically raised poultry products. Further studies involving larger sample sizes over time are needed to better monitor and assess the trend of prevalence and antimicrobial susceptibility among Salmonella serovars in retail chickens.
在这项从2006年10月至2007年9月为期一年的调查中，我们从路易斯安那州巴吞鲁日市27家零售店购买的传统养殖（n = 141）和有机养殖（n = 53）鸡胴体中分离并鉴定了126株沙门氏菌。传统养殖鸡样本中22%分离出沙门氏菌，有机养殖鸡样本中20.8%分离出沙门氏菌。鉴定出8种沙门氏菌血清型；主要血清型包括肯塔基、哈达尔和肠炎。同一鸡样本中的绝大多数分离株具有相同的脉冲场凝胶电泳图谱。所有沙门氏菌分离株对阿米卡星、头孢曲松和环丙沙星敏感；然而，观察到对喹诺酮类药物（7.1%）或广谱头孢菌素（45.2%）的敏感性降低。52.4%的沙门氏菌分离株对多种抗菌药物（两种或更多）耐药。沙门氏菌血清型之间的抗菌药物耐药谱差异很大，并且还取决于分离出它们的鸡的类型。有机鸡样本中的肯塔基沙门氏菌分离株对所测试的11种抗菌药物敏感，而传统养殖鸡中的分离株仅对4种抗菌药物敏感。来自传统养殖鸡的3株肯塔基沙门氏菌分离株具有多重耐药表型MDR - AmpC。本研究结果提供了该地区零售鸡中沙门氏菌的流行率和抗菌药物耐药性的基线数据，并强调需要实施有效控制措施，以减少传统养殖和有机养殖禽产品中沙门氏菌的污染以及耐抗菌药物沙门氏菌的水平。需要进行进一步的研究，随着时间推移纳入更大的样本量，以更好地监测和评估零售鸡中沙门氏菌血清型的流行率趋势和抗菌药物敏感性。
