Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino - Via Pietro Giuria 9 - 10125, Torino, Italy.
Int J Pharm. 2009 Nov 3;381(2):160-5. doi: 10.1016/j.ijpharm.2009.07.010. Epub 2009 Jul 17.
Dextran nanobubbles were prepared with a dextran shell and a perfluoropentan core in which oxygen was stored. To increase the stability polyvinylpirrolidone was also added to the formulation as stabilizing agent. Rhodamine B was used as fluorescent marker to obtain fluorescent nanobubbles. The nanobubble formulations showed sizes of about 500nm, a negative surface charge and a good capacity of loading oxygen, no hemolytic activity or toxic effect on cell lines. The fluorescent labelled nanobubbles could be internalized in Vero cells. Oxygen-filled nanobubbles were able to release oxygen in different hypoxic solutions at different time after their preparation in in vitro experiments. The oxygen release kinetics could be enhanced after nanobubble insonation with ultrasound at 2.5MHz. The oxygen-filled nanobubble formulations might be proposed for therapeutic applications in various diseases.
葡聚糖纳米气泡由葡聚糖外壳和全氟戊烷核心组成，其中储存了氧气。为了提高稳定性，还向配方中添加了聚乙烯吡咯烷酮作为稳定剂。罗丹明 B 被用作荧光标记物以获得荧光纳米气泡。纳米气泡制剂的粒径约为 500nm，具有负表面电荷和良好的载氧能力，对细胞系无溶血活性或毒性作用。荧光标记的纳米气泡可以被 Vero 细胞内化。在体外实验中，制备后的不同缺氧溶液中，充氧纳米气泡能够在不同时间释放氧气。用 2.5MHz 的超声波对纳米气泡进行超声处理后，氧气释放动力学可以得到增强。充氧纳米气泡制剂可能会被提议用于各种疾病的治疗应用。
