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核糖开关对氨基酸的识别与基因调控。

Amino acid recognition and gene regulation by riboswitches.

作者信息

Serganov Alexander, Patel Dinshaw J

机构信息

Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

出版信息

Biochim Biophys Acta. 2009 Sep-Oct;1789(9-10):592-611. doi: 10.1016/j.bbagrm.2009.07.002. Epub 2009 Jul 18.

DOI:10.1016/j.bbagrm.2009.07.002
PMID:19619684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3744886/
Abstract

Riboswitches specifically control expression of genes predominantly involved in biosynthesis, catabolism and transport of various cellular metabolites in organisms from all three kingdoms of life. Among many classes of identified riboswitches, two riboswitches respond to amino acids lysine and glycine to date. Though these riboswitches recognize small compounds, they both belong to the largest riboswitches and have unique structural and functional characteristics. In this review, we attempt to characterize molecular recognition principles employed by amino acid-responsive riboswitches to selectively bind their cognate ligands and to effectively perform a gene regulation function. We summarize up-to-date biochemical and genetic data available for the lysine and glycine riboswitches and correlate these results with recent high-resolution structural information obtained for the lysine riboswitch. We also discuss the contribution of lysine riboswitches to antibiotic resistance and outline potential applications of riboswitches in biotechnology and medicine.

摘要

核糖开关专门控制主要参与生物合成、分解代谢以及来自生命三界的生物体中各种细胞代谢物运输的基因表达。在已鉴定出的众多核糖开关类别中，到目前为止有两种核糖开关分别对氨基酸赖氨酸和甘氨酸作出响应。尽管这些核糖开关识别小分子化合物，但它们都属于最大的核糖开关类别，并且具有独特的结构和功能特征。在这篇综述中，我们试图描述氨基酸响应核糖开关用于选择性结合其同源配体并有效执行基因调控功能的分子识别原理。我们总结了赖氨酸和甘氨酸核糖开关的最新生化和遗传数据，并将这些结果与最近获得的赖氨酸核糖开关的高分辨率结构信息相关联。我们还讨论了赖氨酸核糖开关对抗生素耐药性的贡献，并概述了核糖开关在生物技术和医学中的潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/e015a077299d/nihms139210f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/c1ce8fa9cada/nihms139210f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/8277ddf78efc/nihms139210f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/92633fcdb8cf/nihms139210f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/56fff3a7f31e/nihms139210f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/0de6a112b57b/nihms139210f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/e779a50666dd/nihms139210f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/0be60924566d/nihms139210f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/36ccc720843b/nihms139210f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/673e3f22ebb2/nihms139210f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/e015a077299d/nihms139210f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/c1ce8fa9cada/nihms139210f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/8277ddf78efc/nihms139210f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/92633fcdb8cf/nihms139210f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/56fff3a7f31e/nihms139210f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/0de6a112b57b/nihms139210f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/e779a50666dd/nihms139210f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/0be60924566d/nihms139210f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/36ccc720843b/nihms139210f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/673e3f22ebb2/nihms139210f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d937/3744886/e015a077299d/nihms139210f10.jpg

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Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression.使用核苷酸类似物干扰图谱和抑制技术鉴定代谢物-核糖开关相互作用。
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Analysis of the RNA backbone: structural analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension.RNA主链分析：通过在线探针法以及选择性2'-羟基酰化和引物延伸对核糖开关进行结构分析。
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