Madhaiyan Munusamy, Poonguzhali Selvaraj, Kwon Soon-Wo, Sa Tong-Min
Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2904-8. doi: 10.1099/ijs.0.009811-0. Epub 2009 Jul 23.
Three facultative methylotrophic bacterial strains, designated CBMB127(T), CBMB145 and CBMB147, were isolated from the rhizosphere soil of rice and characterized. The strains produced indole-3-acetic acid and siderophores, had 1-aminocyclopropane-1-carboxylate deaminase activity and sulfur oxidation property and also methanol dehydrogenase. Phylogenetic analysis based on the 16S rRNA and methanol dehydrogenase (mxaF) gene sequences showed that Methylophilus methylotrophus was their close relative. The results of the phenotypic, phylogenetic and genotypic analyses showed that strains CBMB127(T) and CBMB145, with 99.4 % 16S rRNA gene sequence similarity and 99 % DNA-DNA hybridization, could be distinguished from recognized species of Methylophilus. Therefore strain CBMB127(T) and CBMB145 are considered to represent a novel species of Methylophilus, for which the name Methylophilus rhizosphaerae sp. nov. is proposed, with CBMB127(T) (=KACC 13099(T)=NCCB 100233(T)) as the type strain. Strain CBMB147 represents a novel strain of the species Methylophilus methylotrophus.
从水稻根际土壤中分离并鉴定了三株兼性甲基营养细菌菌株，分别命名为CBMB127(T)、CBMB145和CBMB147。这些菌株能产生吲哚-3-乙酸和铁载体，具有1-氨基环丙烷-1-羧酸脱氨酶活性和硫氧化特性，还具有甲醇脱氢酶。基于16S rRNA和甲醇脱氢酶（mxaF）基因序列的系统发育分析表明，嗜甲基甲基ophilus是它们的近亲。表型、系统发育和基因型分析结果表明，菌株CBMB127(T)和CBMB145的16S rRNA基因序列相似性为99.4%，DNA-DNA杂交率为99%，可与已确认的嗜甲基甲基ophilus物种区分开来。因此，菌株CBMB127(T)和CBMB145被认为代表了嗜甲基甲基ophilus的一个新物种，为此提出了新名称嗜甲基甲基ophilus rhizosphaerae sp. nov.，以CBMB127(T)（=KACC 13099(T)=NCCB 100233(T)）作为模式菌株。菌株CBMB147代表嗜甲基甲基ophilus物种的一个新菌株。
