Mori Koji, Yamaguchi Kaoru, Sakiyama Yayoi, Urabe Tetsuro, Suzuki Ken-ichiro
National Institute of Technology and Evaluation, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2894-8. doi: 10.1099/ijs.0.010033-0. Epub 2009 Jul 23.
An anaerobic, thermophilic, thiosulfate-reducing bacterium, strain AZM16c01(T), isolated from a hot spring in Japan [Mori, K., Sunamura, M., Yanagawa, K., Ishibashi, J., Miyoshi, Y., Iino, T., Suzuki, K. & Urabe, T. (2008). Appl Environ Microbiol 74, 6223-6229] was characterized in detail. The 16S rRNA gene sequence analysis had revealed that strain AZM16c01(T) was the first cultivated representative of the candidate phylum OP5. The cells were multicellular filaments with a single polar flagellum. The strain contained iso-C(17 : 0) as the major fatty acid and menaquinone-8(H(6)), menaquinone-8(H(8)) and menaquinone-8(H(10)) as the respiratory quinones. The G+C content of the genomic DNA of strain AZM16c01(T) was 34.6 mol%. Optimum growth was obtained at 65 degrees C, pH 6.5 and in the absence of NaCl, with a doubling time of 10.6 h. On the basis of the results of phylogenetic analysis based on the 16S rRNA gene sequence and the characterization of the strain in this study, we propose the name Caldisericum exile gen. nov., sp. nov. for strain AZM16c01(T) (=NBRC 104410(T)=DSM 21853(T)). In addition, we propose the new phylum name Caldiserica phyl. nov. for the candidate phylum OP5 represented by C. exile gen. nov., sp. nov., and Caldisericaceae fam. nov., Caldisericales ord. nov. and Caldisericia classis nov.
从日本温泉中分离出的一株厌氧、嗜热、能还原硫代硫酸盐的细菌AZM16c01(T) 进行了详细的特性分析[森，K.，砂村，M.，柳川，K.，石桥，J.，三好，Y.，饭野，T.，铃木，K.和浦部，T.（2008年）。《应用与环境微生物学》74，6223 - 6229]。16S rRNA基因序列分析表明，菌株AZM16c01(T) 是候选门OP5的首个培养代表菌株。细胞为具有单根极生鞭毛的多细胞丝状体。该菌株以异-C(17 : 0) 作为主要脂肪酸，以甲基萘醌-8(H(6))、甲基萘醌-8(H(8)) 和甲基萘醌-8(H(10)) 作为呼吸醌。菌株AZM16c01(T) 基因组DNA的G+C含量为34.6 mol%。在65℃、pH 6.5且无氯化钠的条件下生长最佳，倍增时间为10.6小时。基于16S rRNA基因序列的系统发育分析结果以及本研究中该菌株的特性分析，我们提议将菌株AZM16c01(T)（=NBRC 104410(T)=DSM 21853(T)）命名为Caldisericum exile gen. nov., sp. nov.[新属名：流亡热硫化菌属，新种名：流亡热硫化菌]。此外，我们提议将以C. exile gen. nov., sp. nov.为代表的候选门OP5命名为新门Caldiserica phyl. nov.[新门名：热硫化菌门]，以及新科Caldisericaceae fam. nov.[新科名：热硫化菌科]、新目Caldisericales ord. nov.[新目名：热硫化菌目]和新纲Caldisericia classis nov.[新纲名：热硫化菌纲] 。
