Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.
Acta Biomater. 2010 Jan;6(1):12-20. doi: 10.1016/j.actbio.2009.07.023. Epub 2009 Jul 24.
Human mesenchymal stem cells (hMSCs) have tremendous potential as a cell source for regenerative medicine due to their capacity for differentiation into a wide range of connective tissue cell types. Although significant progress has been made in the identification of defined growth factor conditions to induce lineage commitment, the effect of underlying biomaterial properties on functional differentiation is far less understood. Here we conduct a systematic assessment of the role for surface chemistry on cell growth, morphology, gene expression and function during hMSC commitment along osteogenic, chondrogenic and adipogenic lineages. Using self-assembled monolayers of omega-functionalized alkanethiols on gold as model substrates, we demonstrate that biomaterial surface chemistry differentially modulates hMSC differentiation in a lineage-dependent manner. These results highlight the importance of initial biomaterial surface chemistry on long-term functional differentiation of adult stem cells, and suggest that surface properties are a critical parameter that must be considered in the design of biomaterials for stem cell-based regenerative medicine strategies.
人骨髓间充质干细胞（hMSCs）具有分化为多种结缔组织细胞类型的能力，因此作为再生医学的细胞来源具有巨大的潜力。尽管已经在确定的生长因子条件下取得了显著进展，以诱导谱系承诺，但对潜在生物材料特性对功能分化的影响了解得还很少。在这里，我们系统地评估了表面化学在 hMSC 沿着成骨、软骨和成脂谱系承诺过程中的细胞生长、形态、基因表达和功能中的作用。我们使用金上的ω-功能化烷硫醇自组装单分子层作为模型底物，证明生物材料表面化学以谱系依赖性的方式差异调节 hMSC 的分化。这些结果强调了初始生物材料表面化学对成体干细胞长期功能分化的重要性，并表明表面特性是基于干细胞的再生医学策略中生物材料设计必须考虑的关键参数。
