利用存档组织通过聚合酶链反应检测食蟹猴体内的克氏锥虫
Polymerase chain reaction detection of Trypanosoma cruzi in Macaca fascicularis using archived tissues.
作者信息
Williams Jeff T, Mubiru James N, Schlabritz-Loutsevitch Natalia E, Rubicz Rohina C, VandeBerg John L, Dick Edward J, Hubbard Gene B
机构信息
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78245-0549, USA.
出版信息
Am J Trop Med Hyg. 2009 Aug;81(2):228-34.
Abstract
This study describes conventional and real-time polymerase chain reaction (PCR) methods developed to detect and quantify Trypanosoma cruzi DNA in cynomolgus monkeys (Macaca fascicularis) using formalin-fixed paraffin-embedded blocks archived for periods of up to 6 years. The highest concentration of T. cruzi DNA was found in the myocardium, urinary bladder, stomach, lymph node, adrenal gland, and colon. The concentration of T. cruzi DNA detected in cardiac tissues was 10-100-fold greater than found elsewhere; the mean concentrations of T. cruzi DNA in non-cardiac tissues were otherwise comparable. Trypanosoma cruzi DNA was amplified from cerebrum but not cerebellum or kidney. Successful use of DNA from formalin-fixed, paraffin-embedded blocks is important because most pathology laboratories routinely archive wax blocks. This archived resource can be used for further studies on the prevalence of this disease.
摘要
本研究描述了用于检测和定量食蟹猴（猕猴）体内克氏锥虫DNA的常规和实时聚合酶链反应（PCR）方法，该方法使用保存长达6年的福尔马林固定石蜡包埋块。在心肌、膀胱、胃、淋巴结、肾上腺和结肠中发现了最高浓度的克氏锥虫DNA。在心脏组织中检测到的克氏锥虫DNA浓度比在其他部位高出10至100倍；非心脏组织中克氏锥虫DNA的平均浓度在其他方面具有可比性。克氏锥虫DNA可从大脑中扩增出来，但从小脑或肾脏中无法扩增。成功使用福尔马林固定石蜡包埋块中的DNA很重要，因为大多数病理实验室通常会存档蜡块。这种存档资源可用于对该疾病流行情况的进一步研究。
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