Xu Junju, Tan Ninghua, Zeng Guangzhi, Han Hongjin, Huang Huoqiang, Ji Changjiu, Zhu Meiju, Zhang Yumei
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
Zhongguo Zhong Yao Za Zhi. 2009 Apr;34(8):990-3.
To study the chemical constituents in fruit of Alpinia oxyphylla and their cytotoxicities on cancer cell lines.
Compounds were isolated and purified by various column chromatographic methods. Their structures were determined by physico-chemical properties and spectral analyses. Compound cytotoxicity was assessed by the sulforhodamine B (SRB) assay.
Eight compounds were obtained from Me2CO-H2O (70%) extract of the fruit of A. oxyphylla and their structures were identified as: (9E)-humulene-2, 3; 6, 7-diepoxide (1), 3(12), 7(13), 9(E)-humulatriene-2, 6-diol (2), (-)-oplopanone (3), yakuchinone A (4), yakuchinone B (5), tectochrysin (6), isovanillin (7), (2E, 4E)-6-hydroxy-2, 6-dimethylhepta-2, 4-dienal (8), and the cytotoxicities of compounds 1, 3-5 on cancer cell lines, A549, HT-29 and SGC-7901, were also investigated.
Compounds 1-3, 7, 8 were isolated for the first time from this genus and compounds 1, 3-5 exhibited no cytotoxicity against three cancer cell lines at a concentration of 10 mg x L(-1).
研究益智果实中的化学成分及其对癌细胞系的细胞毒性。
采用多种柱色谱方法分离纯化化合物。通过理化性质和光谱分析确定其结构。采用磺酰罗丹明B（SRB）法评估化合物的细胞毒性。
从益智果实的Me2CO-H2O（70%）提取物中得到8个化合物，其结构鉴定为：（9E）-葎草烯-2,3；6,7-二环氧物（1）、3(12),7(13),9(E)-葎草三烯-2,6-二醇（2）、（-）-蛇床酮（3）、氧化前胡素A（4）、氧化前胡素B（5）、柚皮素（6）、异香草醛（7）、（2E,4E）-6-羟基-2,6-二甲基庚-2,4-二烯醛（8），并研究了化合物1、3 - 5对癌细胞系A549、HT - 29和SGC - 7901的细胞毒性。
化合物1 - 3、7、8为首次从该属植物中分离得到，化合物1、3 - 5在浓度为10 mg·L-1时对三种癌细胞系无细胞毒性。
