[On the enzymatic breakdown of tripolyphosphate and diphosphate in comminuted meat. X. Influence of substrate concentration (author's transl)].

The tripolyphosphatase activity of comminuted bovine muscle increased with rising substrate concentration (up to 0.8% tripolyphosphate in presence of 2% NaCl). Addition of NaCl increased the maximum velocity (Vmax) of the enzymatic hydrolysis, determined by Lineweaver-Burk plots. From the results it is concluded that the product-dissociation is the rate-limiting step in the breakdown of tripolyphosphate. Increasing diphosphate concentrations caused only a small increase in the diphosphatase activity of minced tissue. Addition of NaCl lowered the Vmax of the diphosphatase reaction.