Hamm R, Neraal R
Z Lebensm Unters Forsch. 1977 Jun 30;164(2):98-100. doi: 10.1007/BF01354311.
The tripolyphosphatase activity of comminuted bovine muscle increased with rising substrate concentration (up to 0.8% tripolyphosphate in presence of 2% NaCl). Addition of NaCl increased the maximum velocity (Vmax) of the enzymatic hydrolysis, determined by Lineweaver-Burk plots. From the results it is concluded that the product-dissociation is the rate-limiting step in the breakdown of tripolyphosphate. Increasing diphosphate concentrations caused only a small increase in the diphosphatase activity of minced tissue. Addition of NaCl lowered the Vmax of the diphosphatase reaction.
粉碎后的牛肌肉中的三聚磷酸酶活性随底物浓度升高而增加（在2%氯化钠存在的情况下，三聚磷酸钠浓度最高可达0.8%）。添加氯化钠可提高酶促水解的最大速度（Vmax），这是通过Lineweaver-Burk图测定得出的。从结果可以得出结论，产物解离是三聚磷酸分解过程中的限速步骤。增加二磷酸盐浓度只会使切碎组织的二磷酸酶活性略有增加。添加氯化钠会降低二磷酸酶反应的Vmax。
