Hirsch-Behnam A, Delius H, de Villiers E M
Institut für Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, F.R.G.
Virus Res. 1990 Dec;18(1):81-97. doi: 10.1016/0168-1702(90)90091-o.
HPV 2a is commonly associated with verrucae vulgares, whereas HPV 57 was detected in mucosal lesions of the maxillary sinus and the genital tract, as well as in cutaneous lesions. The complete DNA sequences of HPV 2a and HPV 57 were determined. The HPV 2a genome consists of 7860 base pairs and the HPV 57 genome contains 7861 base pairs. On the nucleotide level an 83% homology between the two sequences could be ascertained. Compared to other HPVs they have a high G/C-content (HPV 2a: 48.8%, HPV 57: 50.1%). The genomic organization of both viruses complies with that of other sequenced HPVs. Significant sequence divergence between the HPV 2a and HPV 57 genomes was found in the long control region (LCR), as well as in the early-late-region (ELR). The latter varies in size between the cutaneous (72 to 103 nucleotides) and the mucosal HPVs (252 to 584 nucleotides). According to the sizes of the ELRs of HPV 2a (377 nucleotides) and HPV 57 (478 nucleotides), as well as DNA sequence comparisons, these two viruses could be grouped with the so-called mucosal HPVs. In a search for possible tissue-specific elements, a common amino acid motif, thr-thr/asp-pro-ala-ile/valile/leu was found in the L2 of all mucosal HPVs, as well as in HPV 2a and 57. The L2 of the cutaneous types contain the motif val-ser/thr-arg-thr-gln-tyr.
HPV 2a通常与寻常疣相关，而HPV 57在上颌窦和生殖道的黏膜病变以及皮肤病变中均有检测到。测定了HPV 2a和HPV 57的完整DNA序列。HPV 2a基因组由7860个碱基对组成，HPV 57基因组包含7861个碱基对。在核苷酸水平上，可确定这两个序列之间有83%的同源性。与其他HPV相比，它们具有较高的G/C含量（HPV 2a：48.8%，HPV 57：50.1%）。这两种病毒的基因组组织与其他已测序的HPV一致。在长控制区（LCR）以及早期-晚期区（ELR）发现HPV 2a和HPV 57基因组之间存在显著的序列差异。后者在皮肤HPV（72至103个核苷酸）和黏膜HPV（252至584个核苷酸）之间大小不同。根据HPV 2a（377个核苷酸）和HPV 57（478个核苷酸）的ELR大小以及DNA序列比较，这两种病毒可归类为所谓的黏膜HPV。在寻找可能的组织特异性元件时，在所有黏膜HPV的L2以及HPV 2a和57中发现了一个共同的氨基酸基序，即苏氨酸-苏氨酸/天冬氨酸-脯氨酸-丙氨酸-异亮氨酸/缬氨酸-异亮氨酸/亮氨酸。皮肤类型的L2含有基序缬氨酸-丝氨酸/苏氨酸-精氨酸-苏氨酸-谷氨酰胺-酪氨酸。
