Forslund O, Hansson B G
Department of Medical Microbiology, Lund University, Malmö University Hospital, Sweden.
J Clin Microbiol. 1996 Apr;34(4):802-9. doi: 10.1128/jcm.34.4.802-809.1996.
The genome of human papillomavirus (HPV) type 70 (HPV 70), isolated from a cervical condyloma, was obtained by cloning overlapping PCR products. By automated DNA sequence analysis, the genome was found to consist of 7,905 bp with a G + C content of 40%. The genomic organization showed the characteristic features shared by other sequenced HPVs. Nucleotide sequence comparison with previously known HPV types demonstrated the closest homology with HPV 68 (82%), HPV 39 (82%), HPV 18 (70%), HPV 45 (70%), and HPV 59 (70%). Comparison with seven other partially sequenced HPV 70 isolates showed homologies of between 100 and 99.5%. Cloning of overlapping PCR products and automated DNA sequence analysis was found to be a feasible method of obtaining full-length sequences of HPVs.
从宫颈湿疣中分离出的人乳头瘤病毒70型（HPV 70）基因组，是通过克隆重叠PCR产物获得的。通过自动化DNA序列分析，发现该基因组由7905个碱基对组成，G + C含量为40%。基因组结构显示出与其他已测序HPV共有的特征。与先前已知HPV类型的核苷酸序列比较表明，与HPV 68（82%）、HPV 39（82%）、HPV 18（70%）、HPV 45（70%）和HPV 59（70%）的同源性最高。与其他七个部分测序的HPV 70分离株比较，同源性在100%至99.5%之间。发现克隆重叠PCR产物和自动化DNA序列分析是获得HPV全长序列的可行方法。
