人乳头瘤病毒L1区域的短序列与临床症状及交叉杂交分组相关。
Short sequence in L1 region of human papillomaviruses correlates with clinical pictures and grouping by cross-hybridization.
作者信息
Iwamoto A, Yoshikawa H, Kitagawa K, Igarashi H, Kawana T, Yoshikura H
机构信息
Department of Bacteriology, Faculty of Medicine, University of Tokyo.
出版信息
Jpn J Cancer Res. 1992 Apr;83(4):315-9. doi: 10.1111/j.1349-7006.1992.tb00107.x.
Abstract
A consensus primer-mediated polymerase chain reaction devised to amplify a short sequence in L1 region (L1-PCR) efficiently detected genital human papillomaviruses (HPVs) in clinical materials. Nucleotide sequencing of the amplified fragment showed that L1-PCR is also applicable to hitherto unsequenced HPVs. By comparing the amplified 210 nucleotides HPVs were classified into six groups, which are consistent with clinical pictures and a grouping based on cross-hybridization under the stringent condition.
摘要
一种旨在有效扩增L1区短序列的共识引物介导的聚合酶链反应（L1-PCR）能在临床样本中高效检测出生殖器人乳头瘤病毒（HPV）。对扩增片段的核苷酸测序表明，L1-PCR也适用于迄今未测序的HPV。通过比较扩增的210个核苷酸，HPV被分为六组，这与临床症状以及在严格条件下基于交叉杂交的分组一致。
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