Zheng Dan, Seferos Dwight S, Giljohann David A, Patel Pinal C, Mirkin Chad A
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, Illinois 60208-3113, USA.
Nano Lett. 2009 Sep;9(9):3258-61. doi: 10.1021/nl901517b.
We demonstrate a composite nanomaterial, termed an aptamer nano-flare, that can directly quantify an intracellular analyte in a living cell. Aptamer nano-flares consist of a gold nanoparticle core functionalized with a dense monolayer of nucleic acid aptamers with a high affinity for adenosine triphosphate (ATP). The probes bind selectively to target molecules and release fluorescent reporters which indicate the presence of the analyte. Additionally, these nanoconjugates are readily taken up by cells where their signal intensity can be used to quantify intracellular analyte concentration. These nanoconjugates are a promising approach for the intracellular quantification of other small molecules or proteins, or as agents that use aptamer binding to elicit a biological response in living systems.
我们展示了一种复合纳米材料，称为适配体纳米荧光探针，它可以直接定量活细胞内的分析物。适配体纳米荧光探针由一个金纳米颗粒核心组成，该核心用对三磷酸腺苷（ATP）具有高亲和力的核酸适配体致密单层进行功能化修饰。这些探针选择性地与目标分子结合，并释放荧光报告分子，以指示分析物的存在。此外，这些纳米缀合物很容易被细胞摄取，其信号强度可用于定量细胞内分析物浓度。这些纳米缀合物是对其他小分子或蛋白质进行细胞内定量的一种有前景的方法，或者作为利用适配体结合在生物系统中引发生物反应的试剂。
