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肠杆菌属葡糖苷水解亚种,从干燥河床土壤中分离得到。

Pedobacter glucosidilyticus sp. nov., isolated from dry riverbed soil.

机构信息

College of Life Sciences, Wuhan University, Wuhan 430072, PR China.

出版信息

Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):229-233. doi: 10.1099/ijs.0.008060-0. Epub 2009 Jul 31.

Abstract

Two Gram-staining-negative, rod-shaped, non-spore-forming bacterial strains, 1-2(T) and 1-4 were isolated from dry riverbed soil collected from the Xietongmen area of Tibet, China. On the basis of 16S rRNA gene sequence similarity, the novel strains were shown to belong to the genus Pedobacter, sharing <95 % sequence similarity with all recognized species of the genus Pedobacter. The major respiratory quinone was MK-7 and the predominant cellular fatty acids were iso-C15 : 0, iso-C(17 : 0) 3-OH and summed feature 3 (comprising iso-C(16 : 1)omega7c and/or C(16 : 1)omega6c). The DNA G+C contents were 37.2-37.6 mol%. Chemotaxonomic data supported the affiliation of the two new isolates to the genus Pedobacter and the results of physiological and biochemical tests confirmed that the new strains differed significantly from the recognized species of the genus Pedobacter. Therefore, the new isolates represent a novel species within the genus Pedobacter, for which the name Pedobacter glucosidilyticus sp. nov. is proposed. The type strain is 1-2(T) (=CCTCC AB 206110(T)=KCTC 22438(T)).

摘要

两株革兰氏阴性、杆状、不产芽孢的细菌菌株 1-2(T)和 1-4 从中国西藏协亭门地区采集的干燥河床土壤中分离出来。基于 16S rRNA 基因序列相似性,新菌株被归类为 Pedobacter 属,与所有已识别的 Pedobacter 属物种的相似度<95%。主要呼吸醌为 MK-7,主要细胞脂肪酸为 iso-C15:0、iso-C(17:0)3-OH 和特征脂肪酸 3(包含 iso-C(16:1)omega7c 和/或 C(16:1)omega6c)。DNA G+C 含量为 37.2-37.6mol%。化学分类学数据支持这两个新分离株属于 Pedobacter 属,生理生化试验的结果证实,新菌株与已识别的 Pedobacter 属物种有显著差异。因此,新分离株代表了 Pedobacter 属中的一个新种,建议将其命名为葡糖苷解 Pedobacter 新种。模式菌株为 1-2(T)(=CCTCC AB 206110(T)=KCTC 22438(T))。

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