Muñoz-Espín Daniel, Daniel Richard, Kawai Yoshikazu, Carballido-López Rut, Castilla-Llorente Virginia, Errington Jeff, Meijer Wilfried J J, Salas Margarita
Instituto de Biología Molecular Eladio Viñuela and Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas-Universidad Autónoma de Madrid, Canto Blanco, 28049 Madrid, Spain.
Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13347-52. doi: 10.1073/pnas.0906465106.
Little is known about the organization or proteins involved in membrane-associated replication of prokaryotic genomes. Here we show that the actin-like MreB cytoskeleton of the distantly related bacteria Escherichia coli and Bacillus subtilis is required for efficient viral DNA replication. Detailed analyses of B. subtilis phage ϕ29 showed that the MreB cytoskeleton plays a crucial role in organizing phage DNA replication at the membrane. Thus, phage double-stranded DNA and components of the ϕ29 replication machinery localize in peripheral helix-like structures in a cytoskeleton-dependent way. Importantly, we show that MreB interacts directly with the ϕ29 membrane-protein p16.7, responsible for attaching viral DNA at the cell membrane. Altogether, the results reveal another function for the MreB cytoskeleton and describe a mechanism by which viral DNA replication is organized at the bacterial membrane.
关于原核生物基因组膜相关复制所涉及的组织或蛋白质，我们了解得很少。在这里，我们表明，亲缘关系较远的细菌大肠杆菌和枯草芽孢杆菌中类似肌动蛋白的MreB细胞骨架是病毒DNA高效复制所必需的。对枯草芽孢杆菌噬菌体ϕ29的详细分析表明，MreB细胞骨架在组织膜上的噬菌体DNA复制中起关键作用。因此，噬菌体双链DNA和ϕ29复制机制的组分以细胞骨架依赖的方式定位于外周螺旋状结构中。重要的是，我们表明MreB直接与负责将病毒DNA附着在细胞膜上的ϕ29膜蛋白p16.7相互作用。总之，这些结果揭示了MreB细胞骨架的另一个功能，并描述了一种在细菌膜上组织病毒DNA复制的机制。
