Landeo-Villanueva Guillermo Ernesto, Salazar-Salvatierra María Elena, Ruiz-Quiroz Julio Reynaldo, Zuta-Arriola Noemi, Jarama-Soto Benjamín, Herrera-Calderon Oscar, Pari-Olarte Josefa Bertha, Loyola-Gonzales Eddie
Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, Lima 15001, Peru.
Institute for Research in Biological Chemistry, Microbiology and Biotechnology "Marco Antonio Garrido Malo", Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Lima 15001, Peru.
Antibiotics (Basel). 2023 Feb 10;12(2):369. doi: 10.3390/antibiotics12020369.
The aim of this study was to evaluate the inhibitory activity of the commercially available essential oils of (spearmint) and (eucalyptus) on ATCC 25175 biofilms in vitro, emulating dental plaque conditions. The composition of the essential oils (EOs) was determined using gas chromatography coupled with mass spectrometry (GC-MS), with the main metabolites being Carvone (57.93%) and Limonene (12.91%) for and 1,8-Cineole (Eucalyptol) (65.83%) for . The inhibitory activity was evaluated using the methods of agar-well diffusion and colorimetric microdilution. The inhibition halos were 18.3 ± 0.47 mm and 27.0 ± 0.82 mm, and the MICs were 1.8484 mg/mL and 1.9168 mg/mL for the EOs of and respectively. The activity against the biofilms was evaluated on a substrate of bovine enamel pieces using a basal mucin medium (BMM) in anaerobic conditions with daily sucrose exposition cycles in order to emulate oral cavity conditions. The EOs were applied in a concentration of 0.5% in a sterile saline vehicle with 1% polysorbate 20. After 72 h of cultivation, a significant reduction was observed ( < 0.001%) on the biofilm biomass, which was evaluated by its turbidity in suspension and using a count of the recoverable organisms with regards to the control. The effects of the Eos were not significantly distinct from each other. The EOs showed antimicrobial activity against both the Streptococcus mutans planktonic and biofilm cultures. Thus, EOs may have great potential for the development of pharmaceutical and sanitary products for oral health.
本研究的目的是评估市售的留兰香和桉树叶精油在体外对变形链球菌ATCC 25175生物膜的抑制活性，模拟牙菌斑环境。使用气相色谱-质谱联用（GC-MS）测定精油（EOs）的成分，留兰香的主要代谢产物为香芹酮（57.93%）和柠檬烯（12.91%），桉树叶的主要代谢产物为1,8-桉叶素（桉油精）（65.83%）。采用琼脂孔扩散法和比色微量稀释法评估抑制活性。留兰香和桉树叶精油的抑菌圈分别为18.3±0.47毫米和27.0±0.82毫米，最低抑菌浓度分别为1.8484毫克/毫升和1.9168毫克/毫升。为模拟口腔环境，在厌氧条件下，使用基础粘蛋白培养基（BMM），每天进行蔗糖暴露循环，在牛牙釉质片底物上评估对生物膜的活性。精油以0.5%的浓度应用于含有1%聚山梨酯20的无菌生理盐水载体中。培养72小时后，观察到生物膜生物量显著减少（<0.001%），通过悬浮液中的浊度以及与对照相比可培养微生物的计数来评估生物膜生物量。两种精油的效果没有显著差异。这些精油对变形链球菌的浮游菌和生物膜培养物均显示出抗菌活性。因此，精油在开发口腔健康的医药和卫生产品方面可能具有巨大潜力。
