Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Jun;60(Pt 6):1256-1260. doi: 10.1099/ijs.0.012856-0. Epub 2009 Aug 7.
A novel actinomycete was isolated from soil of a rock surface collected from the peak of Darangshi Oreum (Small Mountain) in Jeju, Republic of Korea. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain DLS-44(T) belonged to the genus Dactylosporangium, with the type strains of Dactylosporangium roseum (99.1 % sequence similarity) and Dactylosporangium fulvum (99.0 %) as the nearest phylogenetic relatives. Substrate mycelium was abundant, irregularly branched, twisted and vivid orange-yellow in colour. Aerial mycelium was not produced on most media tested. Finger-shaped sporangia and globose bodies were formed directly from the vegetative mycelium. The combination of morphological and chemotaxonomic characteristics supported assignment of the actinomycete to the genus Dactylosporangium. Strain DLS-44(T) could be distinguished clearly from all type strains of the genus based on its physiological properties (utilization of methyl alpha-d-mannoside and glycerol, nitrate reduction and growth at 20 degrees C and pH 9.1) and some chemotaxonomic characteristics (absence of unsaturated fatty acids). DNA-DNA relatedness values between strain DLS-44(T) and its closest phylogenetic relatives were 12.2-14.8 % with D. roseum DSM 43916(T) and 2.5-3.6 % with D. fulvum IMSNU 22055(T). On the basis of phenotypic, phylogenetic and DNA-DNA hybridization data, strain DLS-44(T) represents a novel species of the genus Dactylosporangium, for which the name Dactylosporangium darangshiense sp. nov. is proposed. The type strain is strain DLS-44(T) (=KCTC 19560(T) =DSM 45260(T)).
一株新的放线菌是从韩国济州岛的 Darangshi Oreum（小山）山顶的岩石表面土壤中分离得到的。基于 16S rRNA 基因序列的系统发育分析表明，菌株 DLS-44(T) 属于 Dactylosporangium 属，与 Dactylosporangium roseum（99.1%序列相似性）和 Dactylosporangium fulvum（99.0%序列相似性）的模式菌株最为相近。基内菌丝体丰富，不规则分枝，扭曲，颜色鲜艳的橙黄色。在大多数测试的培养基上都没有产生气生菌丝体。指状孢子囊和球形小体直接从营养菌丝体形成。形态学和化学分类特征的结合支持将放线菌归入 Dactylosporangium 属。菌株 DLS-44(T) 可通过其生理特性（利用甲基 alpha-d-甘露糖苷和甘油、硝酸盐还原和在 20°C 和 pH9.1 下生长）和一些化学分类特征（不饱和脂肪酸缺失）与属内所有模式菌株明显区分开来。菌株 DLS-44(T) 与最接近的系统发育亲缘菌株之间的 DNA-DNA 同源性值为 12.2-14.8%（与 D. roseum DSM 43916(T)）和 2.5-3.6%（与 D. fulvum IMSNU 22055(T)）。基于表型、系统发育和 DNA-DNA 杂交数据，菌株 DLS-44(T) 代表 Dactylosporangium 属的一个新种，命名为 Dactylosporangium darangshiense sp. nov.。模式菌株为 DLS-44(T) 菌株（=KCTC 19560(T)=DSM 45260(T)）。
