Department of Applied Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
BIOTEC Culture Collection, BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.
Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1200-1205. doi: 10.1099/ijs.0.016378-0. Epub 2009 Aug 10.
Morphological and chemotaxonomic characterization of actinomycete strain MW2-25(T), isolated from tropical forest soil in Nakhon Sawan Province, Thailand, clearly demonstrated that this strain belongs to the genus Dactylosporangium. Phylogenetic analysis using 16S rRNA gene sequences also indicated that this strain should be classified in the genus Dactylosporangium and showed that the closest relative was Dactylosporangium aurantiacum IFO 12592(T) (99.3 % sequence similarity). DNA-DNA hybridization values and some physiological and biochemical properties indicated that this strain could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Dactylosporangium maewongense sp. nov. is proposed. The type strain is MW2-25(T) (=BCC 34832(T)=JCM 15933(T)).
从泰国那空沙旺省热带森林土壤中分离到的放线菌菌株 MW2-25(T)，通过形态学和化学分类学特征的研究，明确其属于游动放线菌属。16S rRNA 基因序列的系统发育分析也表明，该菌株应归类于游动放线菌属，与橙色游动放线菌IFO 12592(T)（相似度为 99.3%）最为相近。DNA-DNA 杂交值和一些生理生化特性表明，该菌株与亲缘关系最近的菌株能够明显区分开来。基于这些表型和基因型数据，该菌株代表了一个新的种，将其命名为游动放线菌麦旺亚种，其模式菌株为 MW2-25(T)(=BCC 34832(T)=JCM 15933(T))。
