College of Life Sciences, Capital Normal University, Beijing 100048, PR China.
College of Public Health, Shanxi Medical University, Taiyuan 030001, PR China.
Int J Syst Evol Microbiol. 2010 Jun;60(Pt 6):1266-1270. doi: 10.1099/ijs.0.012179-0. Epub 2009 Aug 10.
Four Gram-stain-positive, aerobic or facultatively anaerobic, motile, endospore-forming, rod-shaped bacteria, designated strains FeL05(T), FeL11, Fek19 and Fek21, were isolated from seeds of hybrid rice (Oryza sativa L. Jinyou 611), and their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four strains were members of the genus Paenibacillus. They showed 95.4 % sequence similarity or less with strains of other Paenibacillus species. The G+C content of strain FeL05(T) was found to be 53.3 mol%. Its predominant respiratory quinone was MK-7. The predominant cellular fatty acids were anteiso-C(15 : 0) (61.7 %), C(16 : 0) (10.9 %), iso-C(16 : 0) (7.0 %), anteiso-C(17 : 0) (6.7 %) and iso-C(15 : 0) (5.2 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain FeL05(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus hunanensis sp. nov. is proposed. The type strain is strain FeL05(T) (=ACCC 10718(T) =CGMCC 1.8907(T) =DSM 22170(T)).
从杂交水稻（Oryza sativa L. Jinyou 611）种子中分离到 4 株革兰氏阳性、需氧或兼性厌氧、运动、产芽孢的杆状细菌，分别命名为菌株 FeL05(T)、FeL11、Fek19 和 Fek21。采用多相分类法确定了它们的分类地位。基于 16S rRNA 基因序列的系统发育分析表明，这 4 株菌均属于类芽孢杆菌属。它们与其他类芽孢杆菌属的菌株的序列相似性均小于 95.4%。菌株 FeL05(T)的 G+C 含量为 53.3 mol%。其主要的呼吸醌为 MK-7。主要的细胞脂肪酸为 anteiso-C(15:0)（61.7%）、C(16:0)（10.9%）、iso-C(16:0)（7.0%）、anteiso-C(17:0)（6.7%）和 iso-C(15:0)（5.2%）。根据其表型特征和系统发育的独特性，菌株 FeL05(T)代表了类芽孢杆菌属的一个新种，命名为湖南类芽孢杆菌（Paenibacillus hunanensis sp. nov.）。模式菌株为 FeL05(T) 菌株（=ACCC 10718(T)=CGMCC 1.8907(T)=DSM 22170(T)）。
