Bowd Christopher, Tafreshi Ali, Vizzeri Gianmarco, Zangwill Linda M, Sample Pamela A, Weinreb Robert N
Hamilton Glaucoma Center, Department of Ophthalmology, University of California, San Diego, La Jolla, CA 92037-0946, USA.
J Glaucoma. 2009 Aug;18(6):437-42. doi: 10.1097/IJG.0b013e31818c6f44.
To determine the within-trial and between-trial repeatability of pattern electroretinogram (PERG) measurements in healthy and patient eyes, using a new clinical instrument, the PERGLA.
In all, 70 eyes of 35 healthy individuals (intraocular pressure <22 mm Hg, healthy optic disc by stereophotograph assessment, standard visual fields within normal limits) and 90 eyes of 45 clinic patients (ocular hypertensive, glaucomatous optic neuropathy by stereophotograph assessment and/or repeatable abnormal visual fields) enrolled in the University of California, San Diego Diagnostic Innovations in Glaucoma Study (DIGS) were evaluated. Average mean deviation of patient eyes on standard automated perimetry was -1.81 dB (SD=2.61).
The PERG was recorded using the PERGLA paradigm from both eyes simultaneously twice (ie, 2 trials) by a single operator with electrodes being removed and reattached between recordings. Repeatability of PERG amplitude (microV) and phase (pi rad) between 2 runs within a single trial (within-trial condition) was compared with repeatability between 2 trials (ie, after electrode replacement, between-trial condition) by calculating the coefficients of variability (CVs) and the intraclass correlation coefficients (ICCs) and displaying Bland-Altman plots.
For healthy eyes, amplitude CVs (SD) were 11.5% (11.5) and 9.9% (0.79) for within-trial and between-trial conditions, respectively. ICCs were 0.91 and 0.85. Phase CVs were 1.3% (1.5) (within-trials) and 1.5% (1.4) (between-trials) and ICCs were 0.85 and 0.88. For patient eyes, amplitude CVs (SD) were 12.2% (10.1) and 11.2% (7.5) for within-trial and between-trial conditions, respectively. ICCs were 0.92 and 0.89. Phase CVs were 2.2% (2.2) (within-trials) and 2.4% (2.2) (between-trials) and ICCs were 0.82 and 0.83. Bland-Altman plots indicated good agreement between the repeated recordings and were similar within-trials and between-trials for healthy and patient eyes.
Repeatability of PERGLA recordings is good and is similar within-trials and between-trials for both healthy and patient eyes suggesting this technique is promising for monitoring change over time.
使用新型临床仪器PERGLA，确定健康眼和患眼图形视网膜电图（PERG）测量的试验内和试验间重复性。
共有35名健康个体的70只眼（眼压<22 mmHg，立体照相评估显示健康视盘，标准视野在正常范围内）和45名临床患者的90只眼（高眼压症，立体照相评估显示青光眼性视神经病变和/或重复性异常视野）纳入加利福尼亚大学圣地亚哥分校青光眼诊断创新研究（DIGS）并进行评估。患者眼在标准自动视野计上的平均平均偏差为-1.81 dB（标准差=2.61）。
由一名操作人员使用PERGLA范式同时对双眼进行两次PERG记录（即2次试验），记录之间移除并重新连接电极。通过计算变异系数（CV）和组内相关系数（ICC）并绘制布兰德-奥特曼图，比较单次试验内两次测量之间（试验内情况）以及两次试验之间（即电极更换后，试验间情况）PERG振幅（微伏）和相位（π弧度）的重复性。
对于健康眼，试验内和试验间情况下振幅CV（标准差）分别为11.5%（11.5）和9.9%（0.79）。ICC分别为0.91和0.85。相位CV分别为1.3%（1.5）（试验内）和1.5%（1.4）（试验间），ICC分别为0.85和0.88。对于患眼，试验内和试验间情况下振幅CV（标准差）分别为12.2%（10.1）和11.2%（7.5）。ICC分别为0.92和0.89。相位CV分别为2.2%（2.2）（试验内）和2.4%（2.2）（试验间），ICC分别为0.82和0.83。布兰德-奥特曼图表明重复记录之间一致性良好，且健康眼和患眼在试验内和试验间情况相似。
PERGLA记录的重复性良好，且健康眼和患眼在试验内和试验间情况相似，这表明该技术在监测随时间变化方面具有前景。
