Ando Y, Kimura H, Miwata H, Kudo T, Shibata M, Morishima T
Department of Pediatrics, Nagoya University School of Medicine, Japan.
J Med Virol. 1993 Oct;41(2):170-3. doi: 10.1002/jmv.1890410214.
Herpes simplex virus (HSV) DNA in the cerebrospinal fluid (CSF) of children with herpes simplex encephalitis (HSE) was quantified and typed using the polymerase chain reaction (PCR) assay. During the acute phase, HSV-DNA was detected in the CSF of 13 patients with HSE, including 5 neonates. A restriction profile of the PCR products cleaved with the restriction enzymes XhoI and BglII showed that 2 neonatal samples were HSV-2, and the remainder were HSV-1. The amount of HSV-DNA in the initial CSF ranged from 10(2)-10(5) copies/ml. A significantly greater number of HSV-DNA copies was detected in neonates than in older children (mean 3.9 vs. 2.5, log 10 copies/ml p < .05). Except for one patient, the amount of HSV-DNA decreased gradually with acyclovir therapy. These results show that a quantitative PCR assay is applicable not only to the diagnosis of HSE but also for monitoring the response to antiviral drugs.
采用聚合酶链反应（PCR）分析法对单纯疱疹性脑炎（HSE）患儿脑脊液（CSF）中的单纯疱疹病毒（HSV）DNA进行定量和分型。在急性期，13例HSE患者的脑脊液中检测到HSV-DNA，其中包括5例新生儿。用限制性内切酶XhoI和BglII切割PCR产物后的限制性图谱显示，2份新生儿样本为HSV-2，其余为HSV-1。初始脑脊液中HSV-DNA的含量范围为10(2)-10(5)拷贝/毫升。新生儿中检测到的HSV-DNA拷贝数明显多于大龄儿童（平均3.9对2.5，log 10拷贝/毫升，p <.05）。除1例患者外，阿昔洛韦治疗后HSV-DNA含量逐渐下降。这些结果表明，定量PCR分析法不仅适用于HSE的诊断，也适用于监测对抗病毒药物的反应。
