Bamborschke S, Porr A, Huber M, Heiss W D
Klinik und Poliklinik für Neurologie und Psychiatrie der Universität zu Köln, Federal Republic of Germany.
J Neurol. 1990 Apr;237(2):73-6. doi: 10.1007/BF00314664.
Herpes simplex virus (HSV) was studied by in situ DNA hybridization with a biotinylated cDNA probe in 56 air-dried methanol-fixed cerebrospinal fluid (CSF) cell preparations which had been collected from 12 patients with herpes simplex encephalitis (HSE) during the previous 5 years. In three additional HSE cases, freshly prepared acetone-fixed CSF cell preparations were available. In all cases, CSF cell preparations were obtained by cytocentrifugation. Herpes simplex virus DNA could be demonstrated in 8 of the 12 HSE cases with methanol-fixed cells (66%) and in all 3 cases with fresh acetone-fixed CSF cells. The earliest CSF sample was available at the onset of symptoms and showed positive DNA hybridization. In three cases hybridization was positive after a clinical course of more than 5 weeks but was usually found in the 1st week of illness before the beginning of specific inthrathecal IgG synthesis. In 54 control cases with other acute inflammatory diseases of the CNS, including 14 cases of varicella-zoster meningitis, no positive hybridization was detected. These findings strongly suggest that in situ hybridization in CSF cells is a reliable tool for the early and rapid diagnosis of HSE, especially at the onset of the disease, when no antibodies can be detected.
采用生物素化的 cDNA 探针，通过原位 DNA 杂交技术对单纯疱疹病毒（HSV）进行了研究。研究对象为 56 份空气干燥、甲醇固定的脑脊液（CSF）细胞标本，这些标本来自于过去 5 年中 12 例单纯疱疹性脑炎（HSE）患者。另外还有 3 例 HSE 患者，可获得新鲜制备的丙酮固定的 CSF 细胞标本。所有病例的 CSF 细胞标本均通过细胞离心法获得。在 12 例 HSE 患者中，8 例（66%）甲醇固定细胞标本检测到单纯疱疹病毒 DNA，3 例新鲜丙酮固定的 CSF 细胞标本均检测到该病毒 DNA。最早的 CSF 标本在症状出现时即可获得，且 DNA 杂交呈阳性。3 例患者在病程超过 5 周后杂交呈阳性，但通常在疾病第 1 周，即在鞘内特异性 IgG 合成开始之前即可检测到。在 54 例中枢神经系统其他急性炎症性疾病的对照病例中，包括 14 例水痘 - 带状疱疹脑膜炎病例，均未检测到阳性杂交信号。这些结果强烈表明，CSF 细胞原位杂交是早期快速诊断 HSE 的可靠工具，尤其是在疾病发作时，此时无法检测到抗体。
