Water Quality Center, Marquette University, Civil & Environmental Engineering, PO Box 1881, Milwaukee, Wisconsin, USA.
Kleen Test Products, 1611 Sunset Road, Port Washington, WI 53074, USA.
Int J Syst Evol Microbiol. 2010 Jul;60(Pt 7):1570-1576. doi: 10.1099/ijs.0.014977-0. Epub 2009 Aug 14.
Two facultatively anaerobic, budding bacterial strains, designated W1215-PCA4(T) and SRNK-1, were isolated from water from Lake Michigan, USA. The two strains showed identical ERIC-PCR-generated genomic fingerprints and shared 99.9 % 16S rRNA gene sequence similarity. Strain W1215-PCA4(T) showed highest 16S rRNA gene sequence similarities to Labrys monachus VKM B-1479(T) (95.8 %), Labrys methylaminiphilus DSM 16812(T) (95.1 %), Labrys okinawensis MAFF 210191(T) (96.0 %), Labrys miyagiensis G24103(T) (95.4 %), Labrys neptuniae BCRC 17578(T) (95.7 %) and Labrys portucalensis DSM 17916(T) (95.8 %). Data suggested that the two strains were members of a single novel species of the genus Labrys. The major cellular fatty acids of the two isolates were C(18 : 1)omega7c, C(19 : 0) cyclo omega8c and C(16 : 0). Their polar lipid profiles were highly similar to that of Labrys monachus DSM 5896(T). The primary quinone was ubiquinone Q-10, with minor amounts of Q-9 and Q-11. sym-Homospermidine was the predominant polyamine, with putrescine present in moderate amounts. The two strains were identical in terms of their biochemical and physiological traits, but were distinguishable from other species of the genus Labrys. Hence, the description of a novel species in this genus appears to be justified. The name Labrys wisconsinensis sp. nov. is proposed; the type strain is W1215-PCA4(T) (=DSM 19619(T)=NRRL B-51088(T)).
两株兼性厌氧、出芽的细菌菌株，命名为 W1215-PCA4(T) 和 SRNK-1，从美国密歇根湖的水中分离得到。这两株菌的 ERIC-PCR 生成的基因组指纹图谱完全相同，16S rRNA 基因序列相似度为 99.9%。菌株 W1215-PCA4(T) 与 Labrys monachus VKM B-1479(T)（95.8%）、Labrys methylaminiphilus DSM 16812(T)（95.1%）、Labrys okinawensis MAFF 210191(T)（96.0%）、Labrys miyagiensis G24103(T)（95.4%）、Labrys neptuniae BCRC 17578(T)（95.7%）和 Labrys portucalensis DSM 17916(T)（95.8%）的 16S rRNA 基因序列相似度最高。数据表明，这两株菌是 Labrys 属的一个新种的成员。这两个分离株的主要细胞脂肪酸为 C(18 : 1)omega7c、C(19 : 0)cyclo omega8c 和 C(16 : 0)。它们的极性脂谱与 Labrys monachus DSM 5896(T)非常相似。主要醌类为泛醌 Q-10，少量存在 Q-9 和 Q-11。sym-高亚精胺是主要的多胺，腐胺含量中等。这两个菌株在生化和生理特征方面完全相同，但与 Labrys 属的其他种有区别。因此，该属的一个新种的描述似乎是合理的。提议建立一个新的物种，命名为 Labrys wisconsinensis sp. nov.，其模式菌株为 W1215-PCA4(T)（=DSM 19619(T)=NRRL B-51088(T)）。
