Miller Jonathan A, Kalyuzhnaya Marina G, Noyes Emma, Lara Jimmie C, Lidstrom Mary E, Chistoserdova Ludmila
Department of Microbiology, University of Washington, Seattle, WA 98195, USA.
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1247-1253. doi: 10.1099/ijs.0.63409-0.
A new bacterial isolate from a methylamine enrichment culture is described, representing a novel species of facultatively methylotrophic bacteria. The non-motile bacterium is Gram-negative, replicates by budding and does not form endospores. The isolate utilizes methylated amines, as well as a variety of monosaccharides, disaccharides, amino acids, organic acids, aromatic compounds and alcohols as substrates, but does not utilize methanol. Growth factors are not required, although yeast extract stimulates growth. The major components of the fatty acid profile are C(18 : 1)omega7c, C(19 : 0) cyclo and C(16 : 0). The dominant cellular phospholipids are phosphatidyl acid, phosphatidylcholine and phosphatidylethanolamine. The G + C content of the DNA is 65.7 +/- 0.3 mol%. 16S rRNA gene-based phylogenetic analysis revealed that the novel isolate belongs to the alpha-Proteobacteria and is closely related to the only representative of the genus Labrys, Labrys monachus (97.4 % sequence similarity). However, the level of DNA-DNA relatedness with L. monachus is less than 3 %, justifying the placement of this isolate into a novel species of the genus Labrys. The name Labrys methylaminiphilus sp. nov. is proposed (type strain JLW10(T) = ATCC BAA-1080(T) = DSM 16812(T)).
本文描述了一种从甲胺富集培养物中分离得到的新细菌，它代表了一种新型兼性甲基营养细菌。该非运动性细菌为革兰氏阴性菌，通过出芽进行复制，不形成芽孢。该分离株利用甲基化胺以及多种单糖、双糖、氨基酸、有机酸、芳香化合物和醇类作为底物，但不利用甲醇。尽管酵母提取物能刺激生长，但不需要生长因子。脂肪酸谱的主要成分是C(18 : 1)ω7c、C(19 : 0)环丙烷和C(16 : 0)。主要的细胞磷脂是磷脂酸、磷脂酰胆碱和磷脂酰乙醇胺。DNA的G + C含量为65.7 +/- 0.3 mol%。基于16S rRNA基因的系统发育分析表明，该新分离株属于α-变形菌纲，与Labrys属的唯一代表菌株Labrys monachus密切相关（序列相似性为97.4%）。然而，与L. monachus的DNA-DNA相关性水平低于3%，这证明该分离株应归入Labrys属的一个新物种。提议将其命名为嗜甲基胺Labrys菌（Labrys methylaminiphilus sp. nov.）（模式菌株JLW10(T) = ATCC BAA-1080(T) = DSM 16812(T)）。
