Institute of Nanotechnology and Microsystem Engineering, Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan, Republic of China.
Biomicrofluidics. 2007 May 10;1(2):21503. doi: 10.1063/1.2723669.
Multi-target pathogen detection using heterogeneous medical samples require continuous filtering, sorting, and trapping of debris, bioparticles, and immunocolloids within a diagnostic chip. We present an integrated AC dielectrophoretic (DEP) microfluidic platform based on planar electrodes that form three-dimensional (3D) DEP gates. This platform can continuously perform these tasks with a throughput of 3 muLmin. Mixtures of latex particles, Escherichia coli Nissle, Lactobacillus, and Candida albicans are sorted and concentrated by these 3D DEP gates. Surface enhanced Raman scattering is used as an on-chip detection method on the concentrated bacteria. A processing rate of 500 bacteria was estimated when 100 mul of a heterogeneous colony of 10(7) colony forming units ml was processed in a single pass within 30 min.
使用异质医学样本进行多靶病原体检测需要在诊断芯片内连续过滤、分离和捕获碎片、生物颗粒和免疫胶体。我们提出了一种基于平面电极的集成 AC 介电泳（DEP）微流控平台，该平台形成三维（3D）DEP 门。该平台可以以 3 μL/min 的流速连续完成这些任务。通过这些 3DDEP 门对乳胶颗粒、大肠杆菌 Nissle、乳酸杆菌和白色念珠菌的混合物进行了分选和浓缩。表面增强拉曼散射（SERS）被用作浓缩细菌的片上检测方法。当在 30 分钟内单次通过处理 100μL 10（7）个菌落形成单位/ml 的异质菌落时，估计处理速率为 500 个细菌。
