Miko Angelika, Pries Karin, Haby Sabine, Steege Katja, Albrecht Nadine, Krause Gladys, Beutin Lothar
National Reference Laboratory for Escherichia coli, Federal Institute for Risk Assessment (BfR), Diedersdorfer Weg 1, D-12277 Berlin, Germany.
Appl Environ Microbiol. 2009 Oct;75(20):6462-70. doi: 10.1128/AEM.00904-09. Epub 2009 Aug 21.
A total of 140 Shiga toxin-producing Escherichia coli (STEC) strains from wildlife meat (deer, wild boar, and hare) isolated in Germany between 1998 and 2006 were characterized with respect to their serotypes and virulence markers associated with human pathogenicity. The strains grouped into 38 serotypes, but eight O groups (21, 146, 128, 113, 22, 88, 6, and 91) and four H types (21, 28, 2, and 8) accounted for 71.4% and 75.7% of all STEC strains from game, respectively. Eighteen of the serotypes, including enterohemorrhagic E. coli (EHEC) O26:[H11] and O103:H2, were previously found to be associated with human illness. Genes linked to high-level virulence for humans (stx(2), stx(2d), and eae) were present in 46 (32.8%) STEC strains from game. Fifty-four STEC isolates from game belonged to serotypes which are frequently found in human patients (O103:H2, O26:H11, O113:H21, O91:H21, O128:H2, O146:H21, and O146:H28). These 54 STEC isolates were compared with 101 STEC isolates belonging to the same serotypes isolated from farm animals, from their food products, and from human patients. Within a given serotype, most STEC strains were similar with respect to their stx genotypes and other virulence attributes, regardless of origin. The 155 STEC strains were analyzed for genetic similarity by XbaI pulsed-field gel electrophoresis. O103:H2, O26:H11, O113:H21, O128:H2, and O146:H28 STEC isolates from game were 85 to 100% similar to STEC isolates of the same strains from human patients. By multilocus sequence typing, game EHEC O103:H2 strains were attributed to a clonal lineage associated with hemorrhagic diseases in humans. The results from our study indicate that game animals represent a reservoir for and a potential source of human pathogenic STEC and EHEC strains.
对1998年至2006年期间在德国分离出的140株来自野生动物肉（鹿、野猪和野兔）的产志贺毒素大肠杆菌（STEC）菌株，就其血清型以及与人类致病性相关的毒力标记进行了特征分析。这些菌株分为38个血清型，但8个O群（21、146、128、113、22、88、6和91）和4个H型（21、28、2和8）分别占野味来源的所有STEC菌株的71.4%和75.7%。其中18个血清型，包括肠出血性大肠杆菌（EHEC）O26:[H11]和O103:H2，此前已被发现与人类疾病有关。与人类高致病性相关的基因（stx(2)、stx(2d)和eae）存在于46株（32.8%）来自野味的STEC菌株中。54株来自野味的STEC分离株属于在人类患者中常见的血清型（O103:H2、O26:H11、O113:H21、O91:H21、O128:H2、O146:H21和O146:H28）。将这54株STEC分离株与101株从农场动物、其食品以及人类患者中分离出的相同血清型的STEC分离株进行了比较。在给定的血清型内，大多数STEC菌株在stx基因型和其他毒力属性方面相似，无论其来源如何。通过XbaI脉冲场凝胶电泳对155株STEC菌株进行了遗传相似性分析。来自野味的O103:H2、O26:H11、O113:H21、O128:H2和O146:H28 STEC分离株与来自人类患者的相同菌株的STEC分离株的相似性为85%至100%。通过多位点序列分型，野味来源的EHEC O103:H2菌株属于与人类出血性疾病相关的克隆谱系。我们的研究结果表明，野味动物是人类致病性STEC和EHEC菌株的储存宿主和潜在来源。
