Agro-Biotechnology Research Center, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, 201106 Shanghai, China.
Mol Biol Rep. 2010 Feb;37(2):961-6. doi: 10.1007/s11033-009-9759-0.
Thermal hysteresis proteins (Thps) known as antifreeze proteins for their antifreeze activity, depress the freezing point of water below the melting point in many polar marine fishes, terrestrial arthropods and plants. For the purpose of breeding cold-resistant plants, we designed to introduce the Thp gene into the plants. The physiological and biochemical effect of high-lever expression of the modified Choristoneura fumiferana Thp (ThpI) in Arabidopsis thaliana plants was analyzed. Under low temperature stress, the ThpI transgenic plants exhibited stronger growth than wild-type plants. The elevated cold tolerance of the ThpI over-expressing plants was confirmed by the changes of electrolyte leakage activity, malonyldialdehyde and proline contents. These results preliminarily showed that the Thp possibly be used to enhance the low temperature-tolerant ability of plants.
热滞蛋白（Thps）因其抗冻活性而被称为抗冻蛋白，它们能降低许多极地海洋鱼类、陆地节肢动物和植物中水的冰点，使其低于熔点。为了培育耐寒植物，我们设计将 Thp 基因导入植物。分析了在拟南芥植物中高水平表达修饰的 Choristoneura fumiferana Thp（ThpI）的生理生化效应。在低温胁迫下，ThpI 转基因植物的生长明显强于野生型植物。通过电解质泄漏活性、丙二醛和脯氨酸含量的变化，证实了 ThpI 过表达植株的耐寒性得到提高。这些结果初步表明，Thp 可能被用于增强植物的低温耐受能力。
