Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, 650205 Yunnan, People's Republic of China.
Mol Biol Rep. 2011 Oct;38(7):4475-82. doi: 10.1007/s11033-010-0577-1. Epub 2010 Dec 5.
Rose fragrances play an important role in attracting pollinators and commercial value. However, some genes involved in rose floral scent metabolism are less well understood. Here, wild-type scented rose (WR) and its spontaneous non-scented mutant rose (MR) were analyzed. SPME-GC/MS analysis showed that relative content of 1-ethenyl-4-methoxy-benzene represented was significantly different between WR and MR. We have isolated an EST encoding a MYB family of transcription factor from SSH libraries of the two roses in the previous studies, and designated RhMYB1. In the study, the full-length cDNA of RhMYB1 was identified and characterized by rapid amplification of cDNA ends (RACE). The RhMYB1 full-length cDNA was 1,125 bp containing an 882 bp open reading frame, which encodes a precursor protein of 294 amino acids. Sequence alignments revealed that RhMYB1 shared high similarity with other plants R-type MYB, and RhMYB1 contained a DNA binding domain. Northern blot analysis revealed that RhMYB1 was expressed specifically in flower petal, moreover, the expression level of RhMYB1 in WR increased along with scent emission, and decreased when the scent emission decreased. It is suggested that RhMYB1 might be a putative identification of gene involved in the biosynthesis of rose scent.
玫瑰的香气在吸引传粉者和商业价值方面起着重要作用。然而，一些参与玫瑰花香代谢的基因还不太了解。在这里，对野生型有香味的玫瑰（WR）及其自发的无香味突变体玫瑰（MR）进行了分析。SPME-GC/MS 分析表明，WR 和 MR 之间 1-乙烯基-4-甲氧基-苯的相对含量存在显著差异。我们之前从这两种玫瑰的 SSH 文库中分离出一个编码 MYB 家族转录因子的 EST，并将其命名为 RhMYB1。在这项研究中，通过快速扩增 cDNA 末端（RACE）鉴定并表征了 RhMYB1 的全长 cDNA。RhMYB1 的全长 cDNA 为 1125bp，包含一个 882bp 的开放阅读框，编码 294 个氨基酸的前体蛋白。序列比对表明，RhMYB1 与其他植物的 R 型 MYB 具有高度相似性，并且 RhMYB1 含有一个 DNA 结合域。Northern blot 分析显示，RhMYB1 特异性表达于花瓣中，此外，WR 中 RhMYB1 的表达水平随着香气的释放而增加，当香气释放减少时表达水平降低。这表明 RhMYB1 可能是参与玫瑰香气生物合成的候选基因。
