Biomedical Science Department, DSTL, Porton Down, Salisbury SP4 0JQ, UK.
J Immunol Res. 2014;2014:913632. doi: 10.1155/2014/913632. Epub 2014 Jul 22.
The common marmoset (Callithrix jacchus) is increasingly being utilised as a nonhuman primate model for human disease, ranging from autoimmune to infectious disease. In order to fully exploit these models, meaningful comparison to the human host response is necessary. Commercially available reagents, primarily targeted to human cells, were utilised to assess the phenotype and activation status of key immune cell types and cytokines in naive and infected animals. Single cell suspensions of blood, spleen, and lung were examined. Generally, the phenotype of cells was comparable between humans and marmosets, with approximately 63% of all lymphocytes in the blood of marmosets being T cells, 25% B-cells, and 12% NK cells. The percentage of neutrophils in marmoset blood were more similar to human values than mouse values. Comparison of the activation status of cells following experimental systemic or inhalational infection exhibited different trends in different tissues, most obvious in cell types active in the innate immune response. This work significantly enhances the ability to understand the immune response in these animals and fortifies their use as models of infectious disease.
普通绒猴（Callithrix jacchus）越来越多地被用作人类疾病的非人类灵长类动物模型，范围从自身免疫到传染病。为了充分利用这些模型，有必要与人类宿主的反应进行有意义的比较。商用试剂主要针对人类细胞，用于评估先天和感染动物中关键免疫细胞类型和细胞因子的表型和激活状态。检查了血液、脾脏和肺的单细胞悬浮液。通常，人与绒猴之间的细胞表型具有可比性，血液中的所有淋巴细胞约有 63%是 T 细胞，25%是 B 细胞，12%是 NK 细胞。绒猴血液中的中性粒细胞百分比与人类值更相似，而不是与小鼠值相似。比较实验性全身或吸入性感染后细胞的激活状态在不同组织中表现出不同的趋势，在先天免疫反应活跃的细胞类型中最为明显。这项工作显著增强了理解这些动物中免疫反应的能力，并加强了它们作为传染病模型的使用。
