Chalghoumi R, Marcq C, Théwis A, Portetelle D, Beckers Y
Animal Production Unit, Gembloux Agricultural University, Passage des Déportés, 2 B-5030 Gembloux, Belgium.
Poult Sci. 2009 Oct;88(10):2081-92. doi: 10.3382/ps.2009-00173.
Anti-Salmonella spp. egg yolk antibodies (IgY) simultaneously directed against Salmonella Enteritidis and Salmonella Typhimurium were tested to determine if their inclusion in feed decreased Salmonella spp. cecal colonization in experimentally infected broiler chickens. Egg yolk powder (EYP) was obtained by freeze-drying egg yolks containing anti-Salmonella spp. Immunoglobin Y was included in feed at 5 levels of concentration (0 to 5%). Feeds were formulated to similar nutrient levels and provided for ad libitum intake from d 1 to 28. Three days after initiation of feed treatments (d 4), chickens were co-challenged with equal numbers of Salmonella Enteritidis and Salmonella Typhimurium (2x10(6) cfu/bird). Cecal samples were recovered weekly over the experimental period (d 7 to 28) to enumerate Salmonella spp. The effect of anti-Salmonella spp. IgY feed supplementation on growth performance of infected chickens was also evaluated during the same period. In comparison with the positive control treatment (PC), treatments involving EYP (T1, T2, T3, T4, and T5), whether containing anti-Salmonella spp. IgY or not, significantly improved (P<0.05) the growth performance of challenged chickens, but without reaching the performance levels of nonchallenged chickens (NC1 and NC2). However, no link can be established between the enhancement in growth performance of challenged birds and their contamination levels by Salmonella because in-feed incorporation of EYP had no significant effect on cecal colonization by Salmonella. Furthermore, the comparison of the 5 anti-Salmonella spp. IgY concentration levels in feed did not reveal any anti-Salmonella spp. IgY concentration effect on growth performance and Salmonella cecal colonization. These results suggest that anti-Salmonella spp. IgY would undergo denaturation and degradation after their passage through the animal gastrointestinal tract and reveal that components of EYP other than specific antibodies have a beneficial effect on growth performance.
检测了同时针对肠炎沙门氏菌和鼠伤寒沙门氏菌的抗沙门氏菌属蛋黄抗体（IgY），以确定将其添加到饲料中是否会减少实验感染的肉鸡盲肠中沙门氏菌属的定植。通过冷冻干燥含有抗沙门氏菌属的蛋黄获得蛋黄粉（EYP）。免疫球蛋白Y以5种浓度水平（0%至5%）添加到饲料中。将饲料配制为相似的营养水平，并从第1天至第28天提供给鸡自由采食。开始饲料处理3天后（第4天），用等量的肠炎沙门氏菌和鼠伤寒沙门氏菌（2×10⁶ cfu/只）对鸡进行共同攻毒。在实验期间（第7天至第28天）每周采集盲肠样本以计数沙门氏菌属。在同一时期还评估了添加抗沙门氏菌属IgY的饲料对感染鸡生长性能的影响。与阳性对照处理（PC）相比，涉及EYP的处理（T1、T2、T3、T4和T5），无论是否含有抗沙门氏菌属IgY，均显著改善（P<0.05）了攻毒鸡的生长性能，但未达到未攻毒鸡（NC1和NC2）的性能水平。然而，在攻毒鸡生长性能的提高与其沙门氏菌污染水平之间无法建立联系，因为在饲料中添加EYP对沙门氏菌在盲肠中的定植没有显著影响。此外，对饲料中5种抗沙门氏菌属IgY浓度水平的比较未发现任何抗沙门氏菌属IgY浓度对生长性能和沙门氏菌盲肠定植的影响。这些结果表明，抗沙门氏菌属IgY在通过动物胃肠道后会发生变性和降解，并表明除特异性抗体外，EYP的成分对生长性能具有有益作用。
