Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, Suwon 441-707, Republic of Korea.
Agricultural Microbiology, Department of Agricultural Microbiology, Agricultural College and Research Institute, Madurai, India.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1849-1853. doi: 10.1099/ijs.0.013318-0. Epub 2009 Sep 18.
Two aerobic, Gram-reaction-negative, non-spore-forming bacterial strains, 4M29T and 4M40T, were isolated from cotton composts. The two strains grew in the presence of 0-5% (w/v) NaCl (optimum growth in the absence of NaCl), at pH 6.0-8.0 (optimum, pH 7.0) and at 15-45 degrees C (optimum, 30 degrees C). The strains shared 97.1% 16S rRNA gene sequence similarity. Strains 4M29T and 4M40T showed the next highest levels of 16S rRNA gene sequence similarity to Parapedobacter koreensis Jip14T (95.6 and 94.4%, respectively) and Parapedobacter soli DCY14T (95.2 and 93.8%). The level of DNA-DNA relatedness between strains 4M29T and 4M40T was 38%. The two strains contained iso-C15:0, summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1omega7c) and iso-C17:0 3-OH as major fatty acids, MK-7 as the major respiratory quinone, homospermidine as the only polyamine and phosphatidylethanolamine as the major polar lipid. The DNA G+C contents of strains 4M29T and 4M40T were 47.6 and 48.6 mol%, respectively. On the basis of phylogenetic and phenotypic data, strains 4M29T and 4M40T are considered to represent two novel species of the genus Parapedobacter, for which the names Parapedobacter luteus sp. nov. (type strain 4M29T =KACC 10955T =JCM 15977T) and Parapedobacter composti sp. nov. (type strain 4M40T =KACC 10972T =JCM 15978T) are proposed.
两株需氧、革兰氏反应阴性、非孢子形成的细菌菌株 4M29T 和 4M40T 从棉花堆肥中分离得到。这两株菌在 0-5%（w/v）NaCl 存在下生长（最佳生长条件下无 NaCl），在 pH6.0-8.0（最佳，pH7.0）和 15-45°C（最佳，30°C）下生长。菌株的 16S rRNA 基因序列相似度为 97.1%。菌株 4M29T 和 4M40T 与 Parapedobacter koreensis Jip14T（分别为 95.6%和 94.4%）和 Parapedobacter soli DCY14T（分别为 95.2%和 93.8%）的 16S rRNA 基因序列相似度最高。菌株 4M29T 和 4M40T 之间的 DNA-DNA 相关性为 38%。两株菌含有 iso-C15:0、总和特征 3（包含 iso-C15:0 2-OH 和/或 C16:1ω7c）和 iso-C17:0 3-OH 作为主要脂肪酸，MK-7 作为主要呼吸醌，腐胺作为唯一的多胺和磷脂酰乙醇胺作为主要极性脂。菌株 4M29T 和 4M40T 的 DNA G+C 含量分别为 47.6%和 48.6mol%。基于系统发育和表型数据，菌株 4M29T 和 4M40T 被认为代表 Parapedobacter 属的两个新种，分别命名为 Parapedobacter luteus sp. nov.（模式菌株 4M29T=KACC 10955T=JCM 15977T）和 Parapedobacter composti sp. nov.（模式菌株 4M40T=KACC 10972T=JCM 15978T）。
