sp. nov., a new member of family isolated from sand fly rearing substrate.

A Gram-stain-negative, rod-shaped, non-motile, non-spore-forming, aerobic bacterium, designated type strain SSI9, was isolated from sand fly ( Scopoli; ) rearing substrate and subjected to polyphasic taxonomic analysis. Strain SSI9 contained phosphatidylethanolamine as a major polar lipid, MK-7 as the predominant quinone, and Cω6/Cω, iso-C, iso-C 3-OH and C as the major cellular fatty acids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that SSI9 represents a member of the genus , of the family Sphingobacteriaceae sharing 96.5-88.0 % sequence similarity with other species of the genus . The results of multilocus sequence analysis using the concatenated sequences of the housekeeping genes A, C and L indicated that SSI9 formed a separate branch in the genus . The genome of SSI9 is 5 197 142 bp with a DNA G+C content of 41.8 mol% and encodes 4395 predicted coding sequences, 49 tRNAs, and three complete rRNAs and two partial rRNAs. SSI9 could be distinguished from other species of the genus with validly published names by several phenotypic, chemotaxonomic and genomic characteristics. On the basis of the results of this polyphasic taxonomic analysis, the bacterial isolate represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is SSI9 (=ATCC TSD-210=LMG 31664=NRRL B-65603).