新种堆肥鞘氨醇杆菌，从棉籽壳堆肥中分离得到。
Sphingobacterium composti sp. nov., isolated from cotton-waste composts.
作者信息
Yoo Seung-Hee, Weon Hang-Yeon, Jang Han-Byul, Kim Byung-Yong, Kwon Soon-Wo, Go Seung-Joo, Stackebrandt Erko
机构信息
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Republic of Korea.
出版信息
Int J Syst Evol Microbiol. 2007 Jul;57(Pt 7):1590-1593. doi: 10.1099/ijs.0.64948-0.
A Gram-negative, strictly aerobic, short rod-shaped, non-motile bacterial strain designated 4M24(T) was isolated from cotton-waste compost. Analysis of the 16S rRNA gene sequence of strain 4M24(T) revealed that it is a member of the genus Sphingobacterium, sharing 88.5-94.5 % sequence similarity with type strains of the genus Sphingobacterium and being most closely related to Sphingobacterium daejeonense TR6-04(T) (94.5 % sequence similarity) and Sphingobacterium mizutaii ATCC 33299(T) (92.2 % similarity). The major fatty acids of strain 4M24(T) grown on trypticase soy agar medium were summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c; 37.5 %), iso-C(15 : 0) (29.5 %) and iso-C(17 : 0) 3-OH (19.7 %). The G+C content of the genomic DNA was 42.3 mol%. On the basis of phenotypic and genotypic characteristics, strain 4M24(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium composti sp. nov. is proposed. The type strain is 4M24(T) (=KACC 11313(T)=DSM 18850(T)).
从棉花废料堆肥中分离出一株革兰氏阴性、严格需氧、短杆状、无运动性的细菌菌株，命名为4M24(T)。对菌株4M24(T)的16S rRNA基因序列分析表明，它是鞘氨醇杆菌属的成员，与鞘氨醇杆菌属模式菌株的序列相似性为88.5 - 94.5%，与大田鞘氨醇杆菌TR6 - 04(T)（序列相似性94.5%）和水田鞘氨醇杆菌ATCC 33299(T)（相似性92.2%）关系最为密切。在胰蛋白胨大豆琼脂培养基上生长的菌株4M24(T)的主要脂肪酸为总和特征3（异-C(15 : 0) 2-OH和/或C(16 : 1)ω7c；37.5%）、异-C(15 : 0)（29.5%）和异-C(17 : 0) 3-OH（19.7%）。基因组DNA的G+C含量为42.3 mol%。基于表型和基因型特征，菌株4M24(T)代表鞘氨醇杆菌属的一个新物种，为此提出名为堆肥鞘氨醇杆菌（Sphingobacterium composti sp. nov.）的新物种。模式菌株为4M24(T)（=KACC 11313(T)=DSM 18850(T)）。
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