Kim S, Ikeuchi K, Groopman J, Baltimore D
Whitehead Institute, Cambridge, Massachusetts 02142.
J Virol. 1990 Nov;64(11):5600-4. doi: 10.1128/JVI.64.11.5600-5604.1990.
To evaluate the basis of the slow growth of many human immunodeficiency virus strains in monocytes/macrophages, various stages of the virus life cycle have been studied for their possible contribution to viral tropism. Although we found that monocytic U937 cells had a higher percentage of CD4-positive cells than T-lymphoid H9 cells, the human immunodeficiency virus strain grew much less efficiently in the monocytic line. Viral tropism was primarily determined during the early stages of the virus cycle, that is, sometime between binding of the virus to the cell surface and reverse transcription of viral genomic RNA. Once the virus entered the host cell, reverse transcription, use of the long terminal repeat, RNA expression, and production of virus particles was about as efficient in monocytes as in T cells. Thus, during viral entry into the host cell cytoplasm there is a major limiting event that is particularly inefficient in U937 cells and possibly in all monocytes/macrophages.
为评估许多人类免疫缺陷病毒株在单核细胞/巨噬细胞中生长缓慢的原因，对病毒生命周期的各个阶段进行了研究，以探讨其对病毒嗜性的可能影响。尽管我们发现单核细胞系U937细胞中CD4阳性细胞的百分比高于T淋巴细胞系H9细胞，但人类免疫缺陷病毒株在单核细胞系中的生长效率要低得多。病毒嗜性主要在病毒周期的早期阶段决定，即在病毒与细胞表面结合至病毒基因组RNA逆转录之间的某个时间点。一旦病毒进入宿主细胞，逆转录、长末端重复序列的使用、RNA表达以及病毒颗粒的产生在单核细胞中的效率与在T细胞中大致相同。因此，在病毒进入宿主细胞质的过程中存在一个主要的限制事件，该事件在U937细胞中效率极低，可能在所有单核细胞/巨噬细胞中也是如此。
