Goto T, Harada S, Yamamoto N, Nakai M
Department of Microbiology, Osaka Medical College, Japan.
Arch Virol. 1988;102(1-2):29-38. doi: 10.1007/BF01315560.
The ultrastructural features of early events in human immunodeficiency virus (HIV) infection of HTLV-I-carrying MT-2 lymphocytes were investigated by electron microscopy. Within 10 min after virus inoculation at 37 degrees C, the virus entered the cell in two ways; (1) the virus attached to the lymphocyte membrane and the viral core entered the cell after fusion of the viral envelope with the cell membrane, and (2) part of the cell membrane to which the virus was attached became invaginated, the virus became trapped in a phagosome and the viral core entered after the fusion of viral membrane with the vacuolar membrane. Thereafter, some cells were observed to form syncytia with multiple nuclei. When the proportion of anti-HIV antibody-reactive cells present exceeded 90%, virus production was strongly activated, and budding on the cell membrane was frequently observed.
利用电子显微镜研究了携带人嗜T淋巴细胞病毒I型（HTLV-I）的MT-2淋巴细胞感染人类免疫缺陷病毒（HIV）早期事件的超微结构特征。在37℃接种病毒后10分钟内，病毒通过两种方式进入细胞：（1）病毒附着于淋巴细胞膜，病毒包膜与细胞膜融合后病毒核心进入细胞；（2）附着病毒的部分细胞膜内陷，病毒被困于吞噬体中，病毒膜与液泡膜融合后病毒核心进入。此后，观察到一些细胞形成多核巨细胞。当抗HIV抗体反应性细胞的比例超过90%时，病毒产生被强烈激活，且经常观察到细胞膜上有出芽现象。
