State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
National Engineering Laboratory of Biohydrometallurgy, General Research Institute for Nonferrous Metals, Beijing 100088, PR China.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1897-1903. doi: 10.1099/ijs.0.013235-0. Epub 2009 Sep 25.
Two bacterial strains, DNG5T and V3M1T, isolated from forest soil of the Changbai mountains in China, were characterized using a polyphasic approach. Analysis of their 16S rRNA gene sequences indicated that strains DNG5T and V3M1T were phylogenetically related to members of the genus Agrococcus (96.0-98.4% similarity) and Micrococcus (96.7-98.0% similarity), respectively, within the order Actinomycetales. Strains DNG5T and V3M1T were Gram-stain-positive and strictly aerobic and formed yellow colonies on LB agar. Cells of strain DNG5T were short, non-motile rods, 0.4-0.5x0.8-1.0 microm. Strain DNG5T contained MK-10 and MK-11 as the major respiratory quinones and anteiso-C15:0 (49.2%) and iso-C16:0 (22.4%) as the major fatty acids. The diamino acid in the peptidoglycan of strain DNG5T was 2,4-diaminobutyric acid and the murein was of the acetyl type. Cells of strain V3M1T were cocci, 0.6-0.7 microm in diameter. The cell-wall peptidoglycan of strain V3M1T contained the amino acids lysine, glutamic acid, alanine and glycine. Strain V3M1T contained MK-7, MK-7(H2), MK-8 and MK-8(H2) as respiratory quinones and anteiso-C15:0 (78.2%) and iso-C15:0 (13.1%) as the major cellular fatty acids. The DNA G+C contents of strains DNG5T and V3M1T were 75.9 and 67.2 mol%, respectively. The DNA-DNA relatedness of strain DNG5T to Agrococcus jejuensis DSM 22002T, A. jenensis JCM 9950T, A. baldri JCM 12132T and A. citreus JCM 12398T was 58.3, 43.9, 36.1 and 54.1%, respectively. The DNA-DNA relatedness of strain V3M1T to Micrococcus luteus CGMCC 1.2299T, M. antarcticus CGMCC 1.2373T and M. lylae CGMCC 1.2300T was 57.5, 45.4 and 39.0%, respectively. Combining phenotypic and genotypic traits, strain DNG5T represents a novel species of the genus Agrococcus, for which the name Agrococcus terreus sp. nov. is proposed, with DNG5T (=CGMCC 1.6960T =NBRC 104260T) as the type strain. Strain V3M1T represents a novel species of the genus Micrococcus, for which the name Micrococcus terreus sp. nov. is proposed, with V3M1T (=CGMCC 1.7054T =NBRC 104258T) as the type strain.
两株细菌菌株，DNG5T 和 V3M1T，从中国长白山森林土壤中分离出来，采用多相方法进行了特征描述。16S rRNA 基因序列分析表明，菌株 DNG5T 和 V3M1T 分别与 Agrococcus 属（96.0-98.4%相似性）和 Micrococcus 属（96.7-98.0%相似性）的成员密切相关，在放线菌目中。菌株 DNG5T 和 V3M1T 革兰氏阳性，严格需氧，在 LB 琼脂上形成黄色菌落。菌株 DNG5T 的细胞为短的、非运动的棒状，0.4-0.5x0.8-1.0 微米。菌株 DNG5T 含有 MK-10 和 MK-11 作为主要呼吸醌，anteiso-C15:0（49.2%）和 iso-C16:0（22.4%）作为主要脂肪酸。菌株 DNG5T 肽聚糖中的二氨基氨基酸为 2,4-二氨基丁酸，肽聚糖为乙酰型。菌株 V3M1T 的细胞为球菌，直径 0.6-0.7 微米。菌株 V3M1T 的细胞壁肽聚糖含有赖氨酸、谷氨酸、丙氨酸和甘氨酸。菌株 V3M1T 含有 MK-7、MK-7(H2)、MK-8 和 MK-8(H2)作为呼吸醌，anteiso-C15:0（78.2%）和 iso-C15:0（13.1%）作为主要细胞脂肪酸。菌株 DNG5T 和 V3M1T 的 DNA G+C 含量分别为 75.9 和 67.2 mol%。菌株 DNG5T 与 Agrococcus jejuensis DSM 22002T、A. jenensis JCM 9950T、A. baldri JCM 12132T 和 A. citreus JCM 12398T 的 DNA-DNA 同源性分别为 58.3%、43.9%、36.1%和 54.1%。菌株 V3M1T 与 Micrococcus luteus CGMCC 1.2299T、M. antarcticus CGMCC 1.2373T 和 M. lylae CGMCC 1.2300T 的 DNA-DNA 同源性分别为 57.5%、45.4%和 39.0%。综合表型和基因型特征，菌株 DNG5T 代表了一个新的 Agrococcus 属种，命名为 Agrococcus terreus sp. nov.，其模式菌株为 DNG5T（=CGMCC 1.6960T =NBRC 104260T）。菌株 V3M1T 代表了一个新的 Micrococcus 属种，命名为 Micrococcus terreus sp. nov.，其模式菌株为 V3M1T（=CGMCC 1.7054T =NBRC 104258T）。
