一种用于生理流动中细胞的光学操控技术。
An optical-manipulation technique for cells in physiological flows.
作者信息
Zhang Hu, Chen Neng H, El Haj Alicia, Liu Kuo-Kang
出版信息
J Biol Phys. 2010 Mar;36(2):135-43. doi: 10.1007/s10867-009-9176-6. Epub 2009 Sep 29.
Abstract
We have developed a technique to manipulate human red blood cells (RBCs) in hydrodynamic flows. This method applies optical tweezers to trap and move microbead-attached RBCs in a liquid medium at various speeds, while it significantly minimizes laser heating and photon-induced stress for normal operation with laser-trapped cells. Computational fluid dynamics is applied to simulate flow-induced shear stress over the cell membrane and to correlate quantitatively the forces with the cell deformations. RBCs can be manipulated under physiological conditions by this approach, which may open an avenue to design principles for the next generation of cell sorting and delivery.
摘要
我们开发了一种在流体动力学流动中操纵人类红细胞（RBCs）的技术。该方法应用光镊在液体介质中以各种速度捕获并移动附着微珠的红细胞，同时在使用激光捕获细胞进行正常操作时，能显著减少激光加热和光子诱导的应力。应用计算流体动力学来模拟细胞膜上的流动诱导剪切应力，并将力与细胞变形进行定量关联。通过这种方法可以在生理条件下操纵红细胞，这可能为下一代细胞分选和递送的设计原则开辟一条道路。
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