Institute of Science and Technology in Medicine, Keele University, Stoke-on-Trent, UK.
J Tissue Eng. 2012;3(1):2041731412451988. doi: 10.1177/2041731412451988. Epub 2012 Jul 2.
Stem cell therapy may rely on delivery and homing through the vascular system to reach the target tissue. An optical tweezer model has been employed to exert different levels of shear stress on a single non-adherent human bone marrow-derived mesenchymal stem cell to simulate physiological flow conditions. A single-cell quantitative polymerase chain reaction analysis showed that collagen type 1, alpha 2 (COL1A2), heat shock 70-kDa protein 1A (HSPA1A) and osteopontin (OPN) are expressed to a detectable level in most of the cells. After exposure to varying levels of shear stress, there were significant variations in gene transcription levels across human mesenchymal stem cells derived from four individual donors. Significant trend towards upregulation of COL1A2 and OPN gene expression following shear was observed in some donors with corresponding variations in HSPA1A gene expression. The results indicate that shear stress associated with vascular flow may have the potential to significantly direct non-adherent stem cell expression towards osteogenic phenotypic expression. However, our results demonstrate that these results are influenced by the selection process and donor variability.
干细胞治疗可能依赖于通过血管系统的输送和归巢来达到靶组织。已经采用光镊模型对单个非黏附的人骨髓间充质干细胞施加不同水平的剪切力，以模拟生理流动条件。单细胞定量聚合酶链反应分析表明，大多数细胞中可检测到胶原类型 1，α 2（COL1A2），热休克 70kDa 蛋白 1A（HSPA1A）和骨桥蛋白（OPN）的表达。在暴露于不同水平的剪切力后，来自四个个体供体的人骨髓间充质干细胞的基因转录水平存在显著变化。在一些供体中，COL1A2 和 OPN 基因表达在剪切后呈显著上调趋势，HSPA1A 基因表达也相应变化。结果表明，与血管流动相关的剪切力可能具有显著指导非黏附干细胞向成骨表型表达的潜力。然而，我们的结果表明，这些结果受到选择过程和供体变异性的影响。
